A fluorescence polarization-based interaction assay for hypoxia-inducible factor prolyl hydroxylases

Hyunju Cho, Hyunsung Park, Eun Gyeong Yang

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Oxygen-dependent ubiquitination and degradation of hypoxia-inducible factor 1α (HIF-1α) plays a central role in regulating transcriptional responses to hypoxia. This process requires hydroxylation of specific prolines in HIF-1α by HIF prolyl hydroxylase domain (PHD)-containing enzymes, leading to its specific interactions with von Hippel-Lindau protein-Elongin B-Elongin C (VBC). Here we describe a straightforward approach to apply these interactions to measure PHD activities. Employing fluorescently labeled HIF-1α peptides containing hydroxyproline, we developed a quantitative method based on fluorescence polarization for a systematic evaluation of binding of hydroxylated HIF-1α to recombinant VBC. The method was then successfully utilized for measuring the activity of the truncated, purified PHD2. The applicability of the assay was further demonstrated by examining effects of various cofactors and inhibitors for PHD2. The developed homogeneous assay would provide a convenient way of probing the biochemical properties of the HIF-1α-VBC interaction and PHDs, and of screening modulators for the interaction as well as the enzyme.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number1
DOIs
StatePublished - 11 Nov 2005

Keywords

  • Fluorescence polarization
  • HIF-1α-VBC interaction
  • Hydroxyproline
  • Hypoxia-inducible factor
  • Prolyl hydroxylase
  • VBC
  • von Hippel-Lindau protein

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