Amplified microelectrode recordings of neuron clusters in a three dimensional cell culture chip

M. Son, I. Choi, S. Chung, J. Y. Kang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Amplified neuronal signals ean be recorded by microelectrode array (MEA) in microfluidic channels where neurons were cultured in 3D. MEA was located at the bottom of channels where only axons, isolated from somas by collagen blocks, were grown. The impedance of microchannels is higher than that of open chambers, creating enhanced signals at electrodes compared to simple chambers. The first signals were recorded at 3 weeks during the 5 weeks of culture period. The magnitude of signals was increased at week 5 and decreased afterwards. This chip is expected to provide a useful tool for long-term cultures of neurons that can observe axon growth as well as amplified action potentials.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages413-415
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • 3D culture
  • Long-term neuron culture
  • MEA
  • Microchannels
  • Neuronal signals

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