Analyses of the TCR repertoire of MHC class II-restricted innate CD4+ Tcells

Byung Hyun Kang, Hye Sook Min, You Jeong Lee, Bomi Choi, Eun Ji Kim, Jonghoon Lee, Jeong Rae Kim, Kwang Hyun Cho, Tae Jin Kim, Kyeong Cheon Jung, Seong Hoe Park

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7 Scopus citations


Analysis of the T-cell receptor (TCR) repertoire of innate CD4+ T cells selected by major histocompatibility complex (MHC) class II-dependent thymocyte–thymocyte (T-T) interaction (T-T CD4+ T cells) is essential for predicting the characteristics of the antigens that bind to these T cells and for distinguishing T-T CD4+ T cells from other types of innate T cells. Using the TCRmini Tg mouse model, we show that the repertoire of TCRα chains in T-T CD4+ T cells was extremely diverse, in contrast to the repertoires previously described for other types of innate T cells. The TCRα chain sequences significantly overlapped between T-T CD4+ T cells and conventional CD4+ T cells in the thymus and spleen. However, the diversity of the TCRα repertoire of T-T CD4+ T cells seemed to be restricted compared with that of conventional CD4+ T cells. Interestingly, the frequency of the parental OT-II TCRα chains was significantly reduced in the process of T-T interaction. This diverse and shifted repertoire in T-T CD4+ T cells has biological relevance in terms of defense against diverse pathogens and a possible regulatory role during peripheral T-T interaction.

Original languageEnglish
Article numbere154
JournalExperimental and Molecular Medicine
Issue number3
StatePublished - Mar 2015


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