Antioxidants prevent particulate matter-induced senescence of lung fibroblasts

Sein Jin, Sung Jin Yoon, Na Young Jung, Wang Sik Lee, Jinyoung Jeong, Young Jun Park, Wantae Kim, Doo Byoung Oh, Jinho Seo

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Particulate matter (PM) contributes to human diseases, particularly lung disease; however, the molecular mechanism of its action is yet to be determined. Herein, we found that prolonged PM exposure induced the cellular senescence of normal lung fibroblasts via a DNA damage-mediated response. This PM-induced senescence (PM-IS) was only observed in lung fibroblasts but not in A549 lung adenocarcinoma cells. Mechanistic analysis revealed that reactive oxygen species (ROS) activate the DNA damage response signaling axis, increasing p53 phosphorylation, ultimately leading to cellular senescence via an increase in p21 expression without affecting the p16-pRB pathway. A549 cells, instead, were resistant to PM-IS due to the PM-induced ROS production suppression. Water-soluble antioxidants, such as vitamin C and N-Acetyl Cysteine, were found to alleviate PM-IS by suppressing ROS production, implying that antioxidants are a promising therapeutic intervention for PM-mediated lung pathogenesis.

Original languageEnglish
Article numbere14179
JournalHeliyon
Volume9
Issue number3
DOIs
StatePublished - Mar 2023

Keywords

  • Antioxidants
  • Cellular senescence
  • DNA damage Response
  • Particulate matter
  • Reactive oxygen species

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