c-Jun amino-terminal kinase is regulated by Gα12/Gα13 and obligate for differentiation of P19 embryonal carcinoma cells by retinoic acid

Eek Hoon Jho, Roger J. Davis, Craig C. Malbon

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63 Scopus citations

Abstract

Retinoic acid induces P19 mouse embryonal carcinoma cells to differentiate to endoderm and increases expression of the heterotrimeric G- protein subunits Gα12 and Gα13. Retinoic acid was found to induce differentiation and sustained activation of c-Jun amino-terminal kinase, but not of ERK1,2 or of p38 mitogen-activated protein kinases. Much like retinoic acid, expression of constitutively active forms of Gα12 and Gα13 induced differentiation and constitutive activation of c-Jun amino-terminal kinase. Expression of the dominant negative form of c-Jun amino-terminal kinase 1 blocked both the activation of c-Jun amino-terminal kinase and the induction of endodermal differentiation in the presence of retinoic acid. These data implicate c-Jun amino-terminal kinase as a downstream element of activation of Gα12 or Gα13 obligate for retinoic acid-induced differentiation.

Original languageEnglish
Pages (from-to)24468-24474
Number of pages7
JournalJournal of Biological Chemistry
Volume272
Issue number39
DOIs
StatePublished - 26 Sep 1997

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