Characterization of the effects of silver nanoparticles on liver cell using HR-MAS NMR spectroscopy

Siwon Kim, Sosun Kim, Sangmi Lee, Bobae Kwon, Jinhee Choi, Jin Won Hyun, Suhkmann Kim

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

AgNPs (silver nanoparticles) has been widely used for the commercial products, which have antimicrobial agent, medical devices, food industry and cosmetics. Despite, AgNPs have been reported as toxic to the mammalian cell, lung, liver, brain and other organs and many researchers have investigated the toxicity of AgNPs. In this study, we investigated toxicity of the AgNPs to the liver cell using metabolomics based on HRMAS NMR (High Resolution Magic Angle Spinning Nuclear Magnetic Resonance) technics, which could apply to the intact tissues or cells, to avoid the sample destruction. Target profiling and multivariative statistical analysis were performed to analyze the 1D 1H spectrum. The results show that the concentrations of many metabolites were affected by the AgNPs in the liver cell. The concentrations of glutathione (GSH), lactate, taurine, and glycine were decreased and most of amino acids, choline analogues, and pyruvate were increased by the AgNPs. Moreover, the levels of the metabolites were recovered upto similar level of metabolites in the normal cell by the pre-treatment of NAC, external antioxidant. The results suggest that the depletion of the GSH by the AgNPs might induce the conversion of lactate and taurine to the pyruvate.

Original languageEnglish
Pages (from-to)2021-2026
Number of pages6
JournalBulletin of the Korean Chemical Society
Volume32
Issue number6
DOIs
StatePublished - 20 Jun 2011

Keywords

  • HR-MAS NMR
  • Metabolomics
  • Silver nanoparticles
  • Toxicity

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