Cloning of the human sodium iodide symporter

P. A. Smanik; Q. Liu; T. L. Furminger; K. Ryu; S. Xing; E. L. Mazzaferri; Sissy M. Jhiang

doi:10.1006/bbrc.1996.1358

Cloning of the human sodium iodide symporter

P. A. Smanik, Q. Liu, T. L. Furminger, K. Ryu, S. Xing, E. L. Mazzaferri, Sissy M. Jhiang

Department of Life Science

Research output: Contribution to journal › Article › peer-review

470 Scopus citations

Abstract

The iodide concentrating activity of the thyroid gland is essential to the production of thyroid hormone and also provides a mechanism for the treatment of thyroid cancer by radioiodine ablation. We report here the nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which mediates the iodide uptake activity in the thyroid gland. An open reading frame of 1929 nucleotides encodes a protein of 643 amino acids with 84% identity to the rat NIS (rNIS). Transient expression of the hNIS cDNA conferred perchlorate-sensitive iodide uptake to a nonthyroid cell line, COS-7. The expression of hNIS was detected at variable levels in papillary thyroid carcinoma tissues but not in any of the thyroid carcinoma cell lines that have lost the iodide uptake activity.

Original language	English
Pages (from-to)	339-345
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	226
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1996.1358
State	Published - 13 Sep 1996

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AU - Smanik, P. A.

AU - Liu, Q.

AU - Furminger, T. L.

AU - Ryu, K.

AU - Xing, S.

AU - Mazzaferri, E. L.

AU - Jhiang, Sissy M.

PY - 1996/9/13

Y1 - 1996/9/13

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AB - The iodide concentrating activity of the thyroid gland is essential to the production of thyroid hormone and also provides a mechanism for the treatment of thyroid cancer by radioiodine ablation. We report here the nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which mediates the iodide uptake activity in the thyroid gland. An open reading frame of 1929 nucleotides encodes a protein of 643 amino acids with 84% identity to the rat NIS (rNIS). Transient expression of the hNIS cDNA conferred perchlorate-sensitive iodide uptake to a nonthyroid cell line, COS-7. The expression of hNIS was detected at variable levels in papillary thyroid carcinoma tissues but not in any of the thyroid carcinoma cell lines that have lost the iodide uptake activity.

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Cloning of the human sodium iodide symporter

Abstract

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