Cultivar Resistance of Korean Breeding Cut-Rose against Crown Gall by Agrobacterium tumefaciens Evaluated by an In Vitro Inoculation

Serah Lim, Se Chul Chun, Jin Won Kim

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Rose crown gall caused by Agrobacterium tumefaciens is a major disease that damages the production of cut-roses in Korea. The effective prevention methods for this disease include the use of resistant varieties. This study was conducted to evaluate the resistance of 58 Korean cultivars and six foreign cultivars to crown gall disease with nodal explants in vitro. Among 180 A. tu-mefaciens strains, pathogenic strain RC12 was selected as an inoculant strain. The strain RC12 was identified based on characteristics of some selective media, patho-genicity test, and polymerase chain reaction analysis. Forty rose cultivars formed tumors on explants inoculated with A. tumefaciens RC12. However, 24 cultivars, including 22 Korean cultivars and 2 foreign cultivars, showed resistance to A. tumefaciens RC12 without forming any tumors. Six cultivars with tumor formation rates of over 30% formed initial tumors within 23 days after inoculation. Six cultivars with low tumor formation rates of around 5% formed initial tumors after 28 days of inoculation. It was found that gall formation rate was highly correlated with the initial gall formation period. Thus, the relationship between the period of gall formation and the rate of gall formation could be useful for assessing resistance to crown gall disease. In vitro inoculation methods could be used to evaluate resistance of cut-rose cultivars to crown gall diseases.

Original languageEnglish
Pages (from-to)220-227
Number of pages8
JournalPlant Pathology Journal
Volume39
Issue number2
DOIs
StatePublished - Apr 2023

Keywords

  • Agrobacterium tumefaciens
  • crown gall
  • in vi-tro inoculation
  • resistance
  • rose

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