TY - JOUR
T1 - Determination of nanomolar levels of reactive oxygen species in microorganisms and aquatic environments using a single nanoparticle-based optical sensor
AU - Kim, Yura
AU - Kim, Youngho
AU - Choi, Jinhee
AU - Kang, Taewook
AU - Choi, Inhee
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/5/15
Y1 - 2017/5/15
N2 - Reactive oxygen species (ROS) are strong oxidants, and have attracted considerable attention in both biological and environmental fields. Although various methods for ROS detection, including optical and electrochemical techniques, have been developed, they still face challenging issues in terms of poor sensitivity, reproducibility, stability, and in vivo applicability. Here, we present a sensitive and selective optical sensor for ROS detection, based on single plasmonic nanoprobes and redox-active cytochrome c (Cyt c)-mediated plasmon resonance energy transfer. By measuring the spectral changes of plasmonic nanoprobes, derived from the unique molecular absorption of Cyt c in accordance with the redox state, calibration curves for H2O2, a representative ROS, in various media were obtained over a wide concentration range from 100 mM to 1 nM. Limit of detection and limit of quantification in accordance with the used medium were determined from 8.3 to 12.8 nM and from 27.6 to 42.7 nM, respectively. Selectivity coefficients for major interfering solutes were much lower than 0.1 indicating a good selectivity for ROS. From the dynamic spectral changes, we sensitively monitored ROS generated in Caenorhabditis elegans (C. elegans) exposed to graphene oxide. Based on the calibration curves, we also determined ROS levels in various aquatic environments, such as river streams and a small pond, as a way of environmental monitoring. We believe that our approach could provide an avenue for achieving dynamic and sensitive monitoring of ROS in toxicological, biological, and environmental fields in the future.
AB - Reactive oxygen species (ROS) are strong oxidants, and have attracted considerable attention in both biological and environmental fields. Although various methods for ROS detection, including optical and electrochemical techniques, have been developed, they still face challenging issues in terms of poor sensitivity, reproducibility, stability, and in vivo applicability. Here, we present a sensitive and selective optical sensor for ROS detection, based on single plasmonic nanoprobes and redox-active cytochrome c (Cyt c)-mediated plasmon resonance energy transfer. By measuring the spectral changes of plasmonic nanoprobes, derived from the unique molecular absorption of Cyt c in accordance with the redox state, calibration curves for H2O2, a representative ROS, in various media were obtained over a wide concentration range from 100 mM to 1 nM. Limit of detection and limit of quantification in accordance with the used medium were determined from 8.3 to 12.8 nM and from 27.6 to 42.7 nM, respectively. Selectivity coefficients for major interfering solutes were much lower than 0.1 indicating a good selectivity for ROS. From the dynamic spectral changes, we sensitively monitored ROS generated in Caenorhabditis elegans (C. elegans) exposed to graphene oxide. Based on the calibration curves, we also determined ROS levels in various aquatic environments, such as river streams and a small pond, as a way of environmental monitoring. We believe that our approach could provide an avenue for achieving dynamic and sensitive monitoring of ROS in toxicological, biological, and environmental fields in the future.
KW - Cytochrome c
KW - Hydrogen peroxide
KW - Optical sensor
KW - Plasmon resonance energy transfer
KW - Plasmonic nanoparticles
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85017010902&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2017.03.012
DO - 10.1016/j.aca.2017.03.012
M3 - Article
C2 - 28390490
AN - SCOPUS:85017010902
SN - 0003-2670
VL - 967
SP - 85
EP - 92
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -