TY - GEN
T1 - Development of a novel biosensor for in-vitro observation of protein behaviors
AU - Choi, Inhee
AU - Lee, Suseung
AU - Hong, Surin
AU - Yang, Young In
AU - Song, Hyeon Don
AU - Yi, Jongheop
AU - Kang, Taewook
PY - 2009
Y1 - 2009
N2 - We demonstrate a novel biosensor for in vitro observation of cell membrane behaviors associated with proteins, adapting a supported lipid bilayer (SLB) that mimics the cellular membrane and using label-free surface plasmon resonance spectroscopy (SPRS). In this study, we focus on the detection of membrane behaviors by the specific types of Cu/Zn-superoxide dismutase (SOD1) species, which have been implicated in the neurodegenerative disease. The biomimetic sensor chip was fabricated by the fusion of unilamellar lipid vesicles on a hydrophilic Au surface for SPRS. After injection of several protein samples, the degradation signal of lipid membrane for specific type of protein species was real-time monitored by SPRS. This sensor allows one to observe in vitro the membrane damage due to the protein aggregations, and, importantly, to further develop biomarkers for injured cells.
AB - We demonstrate a novel biosensor for in vitro observation of cell membrane behaviors associated with proteins, adapting a supported lipid bilayer (SLB) that mimics the cellular membrane and using label-free surface plasmon resonance spectroscopy (SPRS). In this study, we focus on the detection of membrane behaviors by the specific types of Cu/Zn-superoxide dismutase (SOD1) species, which have been implicated in the neurodegenerative disease. The biomimetic sensor chip was fabricated by the fusion of unilamellar lipid vesicles on a hydrophilic Au surface for SPRS. After injection of several protein samples, the degradation signal of lipid membrane for specific type of protein species was real-time monitored by SPRS. This sensor allows one to observe in vitro the membrane damage due to the protein aggregations, and, importantly, to further develop biomarkers for injured cells.
UR - http://www.scopus.com/inward/record.url?scp=77951142236&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2009.5398233
DO - 10.1109/ICSENS.2009.5398233
M3 - Conference contribution
AN - SCOPUS:77951142236
SN - 9781424445486
T3 - Proceedings of IEEE Sensors
SP - 353
EP - 356
BT - IEEE Sensors 2009 Conference - SENSORS 2009
T2 - IEEE Sensors 2009 Conference - SENSORS 2009
Y2 - 25 October 2009 through 28 October 2009
ER -