Development of a novel biosensor for in-vitro observation of protein behaviors

Inhee Choi, Suseung Lee, Surin Hong, Young In Yang, Hyeon Don Song, Jongheop Yi, Taewook Kang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations


We demonstrate a novel biosensor for in vitro observation of cell membrane behaviors associated with proteins, adapting a supported lipid bilayer (SLB) that mimics the cellular membrane and using label-free surface plasmon resonance spectroscopy (SPRS). In this study, we focus on the detection of membrane behaviors by the specific types of Cu/Zn-superoxide dismutase (SOD1) species, which have been implicated in the neurodegenerative disease. The biomimetic sensor chip was fabricated by the fusion of unilamellar lipid vesicles on a hydrophilic Au surface for SPRS. After injection of several protein samples, the degradation signal of lipid membrane for specific type of protein species was real-time monitored by SPRS. This sensor allows one to observe in vitro the membrane damage due to the protein aggregations, and, importantly, to further develop biomarkers for injured cells.

Original languageEnglish
Title of host publicationIEEE Sensors 2009 Conference - SENSORS 2009
Number of pages4
StatePublished - 2009
EventIEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand
Duration: 25 Oct 200928 Oct 2009

Publication series

NameProceedings of IEEE Sensors


ConferenceIEEE Sensors 2009 Conference - SENSORS 2009
Country/TerritoryNew Zealand


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