Development of reverse transcription-competitive polymerase chain reaction method to quantitate the expression levels of human sodium iodide symporter

Kwon Yul Ryu; Molly E. Senokozlieff; Patricia A. Smanik; Mariwil G. Wong; Allan E. Siperstein; Quan Yang Duh; Orlo H. Clark; Ernest L. Mazzaferri; Sissy M. Jhiang

doi:10.1089/thy.1999.9.405

Development of reverse transcription-competitive polymerase chain reaction method to quantitate the expression levels of human sodium iodide symporter

Kwon Yul Ryu, Molly E. Senokozlieff, Patricia A. Smanik, Mariwil G. Wong, Allan E. Siperstein, Quan Yang Duh, Orlo H. Clark, Ernest L. Mazzaferri, Sissy M. Jhiang

Department of Life Science

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The sodium iodide symporter (NIS) is the plasma membrane protein that mediates active iodide uptake into thyroid follicular cells. To investigate whether human NIS (hNIS) mRNA levels in papillary thyroid carcinomas (PCs) correlate with the ability of tumors to concentrate radioiodide, we developed a reverse transcription-competitive polymerase chain reaction (RT-cPCR) method to quantify the hNIS mRNA levels in thyroid tissues. We studied 7 normal thyroid tissues, 8 PCs, and 1 follicular adenoma, hNIS mRNA levels in PCs were generally lower than those found in normal thyroid tissues. The reduced radioiodide concentrating activity of PCs is due, at least in part, to the reduced expression and/or the decreased stability of hNIS mRNA.

Original language	English
Pages (from-to)	405-409
Number of pages	5
Journal	Thyroid
Volume	9
Issue number	4
DOIs	https://doi.org/10.1089/thy.1999.9.405
State	Published - Apr 1999

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title = "Development of reverse transcription-competitive polymerase chain reaction method to quantitate the expression levels of human sodium iodide symporter",

abstract = "The sodium iodide symporter (NIS) is the plasma membrane protein that mediates active iodide uptake into thyroid follicular cells. To investigate whether human NIS (hNIS) mRNA levels in papillary thyroid carcinomas (PCs) correlate with the ability of tumors to concentrate radioiodide, we developed a reverse transcription-competitive polymerase chain reaction (RT-cPCR) method to quantify the hNIS mRNA levels in thyroid tissues. We studied 7 normal thyroid tissues, 8 PCs, and 1 follicular adenoma, hNIS mRNA levels in PCs were generally lower than those found in normal thyroid tissues. The reduced radioiodide concentrating activity of PCs is due, at least in part, to the reduced expression and/or the decreased stability of hNIS mRNA.",

author = "Ryu, {Kwon Yul} and Senokozlieff, {Molly E.} and Smanik, {Patricia A.} and Wong, {Mariwil G.} and Siperstein, {Allan E.} and Duh, {Quan Yang} and Clark, {Orlo H.} and Mazzaferri, {Ernest L.} and Jhiang, {Sissy M.}",

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T1 - Development of reverse transcription-competitive polymerase chain reaction method to quantitate the expression levels of human sodium iodide symporter

AU - Ryu, Kwon Yul

AU - Senokozlieff, Molly E.

AU - Smanik, Patricia A.

AU - Wong, Mariwil G.

AU - Siperstein, Allan E.

AU - Duh, Quan Yang

AU - Clark, Orlo H.

AU - Mazzaferri, Ernest L.

AU - Jhiang, Sissy M.

PY - 1999/4

Y1 - 1999/4

N2 - The sodium iodide symporter (NIS) is the plasma membrane protein that mediates active iodide uptake into thyroid follicular cells. To investigate whether human NIS (hNIS) mRNA levels in papillary thyroid carcinomas (PCs) correlate with the ability of tumors to concentrate radioiodide, we developed a reverse transcription-competitive polymerase chain reaction (RT-cPCR) method to quantify the hNIS mRNA levels in thyroid tissues. We studied 7 normal thyroid tissues, 8 PCs, and 1 follicular adenoma, hNIS mRNA levels in PCs were generally lower than those found in normal thyroid tissues. The reduced radioiodide concentrating activity of PCs is due, at least in part, to the reduced expression and/or the decreased stability of hNIS mRNA.

AB - The sodium iodide symporter (NIS) is the plasma membrane protein that mediates active iodide uptake into thyroid follicular cells. To investigate whether human NIS (hNIS) mRNA levels in papillary thyroid carcinomas (PCs) correlate with the ability of tumors to concentrate radioiodide, we developed a reverse transcription-competitive polymerase chain reaction (RT-cPCR) method to quantify the hNIS mRNA levels in thyroid tissues. We studied 7 normal thyroid tissues, 8 PCs, and 1 follicular adenoma, hNIS mRNA levels in PCs were generally lower than those found in normal thyroid tissues. The reduced radioiodide concentrating activity of PCs is due, at least in part, to the reduced expression and/or the decreased stability of hNIS mRNA.

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Development of reverse transcription-competitive polymerase chain reaction method to quantitate the expression levels of human sodium iodide symporter

Abstract

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