Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus

Jung Kon Kim; Gi Hong An; Seung Hyun Ahn; Youn Ho Moon; Young Lok Cha; Surn Teh Bark; Yong Hwan Choi; Sae Jung Suh; Sang Gyu Seo; Sun Hyung Kim; Bon Cheol Koo

doi:10.1007/s00449-011-0592-1

Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus

Jung Kon Kim, Gi Hong An, Seung Hyun Ahn, Youn Ho Moon, Young Lok Cha, Surn Teh Bark, Yong Hwan Choi, Sae Jung Suh, Sang Gyu Seo, Sun Hyung Kim, Bon Cheol Koo

Department of Environmental Horticulture

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotypes among Miscanthus species. Randomly amplified polymorphic DNA technique was applied for this study and one fragment which is unique to M. sacchariflorus was identified and then sequenced. Based on the specific fragment, one SCAR primer pair designated as MS62-5F and MS62-5R was designed to amplify an approximately 1,000 bp DNA fragment within the sequenced region. Diagnostic PCR was performed using the primer pair. Using this SCAR marker, approximately 1,000 bp and 1,200 bp DNA fragmentswere obtained in M. sacchariflorus and M. sinensis, respectively. Moreover, M. x giganteus was obtained both bands at the same time. The result showed that this SCAR marker can clearly distinguish the M. sacchariflorus, M. sinensis, and M. x giganteus, respectively.

Original language	English
Pages (from-to)	55-59
Number of pages	5
Journal	Bioprocess and Biosystems Engineering
Volume	35
Issue number	1-2
DOIs	https://doi.org/10.1007/s00449-011-0592-1
State	Published - Jan 2012

Keywords

Bioenergy
Miscanthus
Molecular phenotype
RAPD
SCAR marker

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10.1007/s00449-011-0592-1

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title = "Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus",

abstract = "The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotypes among Miscanthus species. Randomly amplified polymorphic DNA technique was applied for this study and one fragment which is unique to M. sacchariflorus was identified and then sequenced. Based on the specific fragment, one SCAR primer pair designated as MS62-5F and MS62-5R was designed to amplify an approximately 1,000 bp DNA fragment within the sequenced region. Diagnostic PCR was performed using the primer pair. Using this SCAR marker, approximately 1,000 bp and 1,200 bp DNA fragmentswere obtained in M. sacchariflorus and M. sinensis, respectively. Moreover, M. x giganteus was obtained both bands at the same time. The result showed that this SCAR marker can clearly distinguish the M. sacchariflorus, M. sinensis, and M. x giganteus, respectively.",

keywords = "Bioenergy, Miscanthus, Molecular phenotype, RAPD, SCAR marker",

author = "Kim, {Jung Kon} and An, {Gi Hong} and Ahn, {Seung Hyun} and Moon, {Youn Ho} and Cha, {Young Lok} and Bark, {Surn Teh} and Choi, {Yong Hwan} and Suh, {Sae Jung} and Seo, {Sang Gyu} and Kim, {Sun Hyung} and Koo, {Bon Cheol}",

year = "2012",

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doi = "10.1007/s00449-011-0592-1",

language = "English",

volume = "35",

pages = "55--59",

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AU - Kim, Jung Kon

AU - An, Gi Hong

AU - Ahn, Seung Hyun

AU - Moon, Youn Ho

AU - Cha, Young Lok

AU - Bark, Surn Teh

AU - Choi, Yong Hwan

AU - Suh, Sae Jung

AU - Seo, Sang Gyu

AU - Kim, Sun Hyung

AU - Koo, Bon Cheol

PY - 2012/1

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N2 - The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotypes among Miscanthus species. Randomly amplified polymorphic DNA technique was applied for this study and one fragment which is unique to M. sacchariflorus was identified and then sequenced. Based on the specific fragment, one SCAR primer pair designated as MS62-5F and MS62-5R was designed to amplify an approximately 1,000 bp DNA fragment within the sequenced region. Diagnostic PCR was performed using the primer pair. Using this SCAR marker, approximately 1,000 bp and 1,200 bp DNA fragmentswere obtained in M. sacchariflorus and M. sinensis, respectively. Moreover, M. x giganteus was obtained both bands at the same time. The result showed that this SCAR marker can clearly distinguish the M. sacchariflorus, M. sinensis, and M. x giganteus, respectively.

AB - The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotypes among Miscanthus species. Randomly amplified polymorphic DNA technique was applied for this study and one fragment which is unique to M. sacchariflorus was identified and then sequenced. Based on the specific fragment, one SCAR primer pair designated as MS62-5F and MS62-5R was designed to amplify an approximately 1,000 bp DNA fragment within the sequenced region. Diagnostic PCR was performed using the primer pair. Using this SCAR marker, approximately 1,000 bp and 1,200 bp DNA fragmentswere obtained in M. sacchariflorus and M. sinensis, respectively. Moreover, M. x giganteus was obtained both bands at the same time. The result showed that this SCAR marker can clearly distinguish the M. sacchariflorus, M. sinensis, and M. x giganteus, respectively.

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Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus

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