Development of transgenic cassava plants expressing IbOr gene by somatic embryogenesis

Sun Ha Kim, Myoung Duck Kim, Sung Chul Park, Jae Cheol Jeong, Haeng Soon Lee, Sang Soo Kwak

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Cassava (Manihot esculenta Crantz) is a useful root crop for food, animal feed and various industrial materials including biofuel. Despite of its importance as an industrial crop, the genetic engineering approaches to manipulate transgenic plant development in cassava are limited. In this study, to develop new cultivar with high level of carotenoids and enhanced tolerance to environmental stresses, sweetpotato IbOr gene involved in accumulation of carotenoids was introduced into an Indonesian IDB high-yielding cassava cultivar under the control of oxidative stress-inducible SWPA2 promoter through Agrobacterium-mediated transformation of friable embryogenic calli. The 19 transgenic lines were successfully generated on the basis of gDNA-PCR and IbOr transcript levels for further characterization in terms of carotenoid contents and environmental stresses. Therefore, IbOr transgenic cassava plants may be developed for enhanced biomass production with high levels of carotenoids on marginal lands.

Original languageEnglish
Pages (from-to)88-92
Number of pages5
JournalJournal of Plant Biotechnology
Issue number2
StatePublished - 1 Jun 2015


  • Carotenoid
  • Cassava
  • IbOr gene
  • Oxidative stress
  • SWPA2 promoter


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