Direct sequencing of RAPD products provides a set of SCAR markers for discrimination of sweet potato cultivars

Hye Min Lee, Young Hoon Park, Tae Hwan Jun, Soon Wook Kwon, In Soo Choi, Yong Chul Kim, Ravi Gupta, Mi Nam Chung, Sun Hyung Kim, Ping Yang, Yiming Wang, Sun Tae Kim

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Sweet potato (Ipomoea batatas L. Lam) is one of the most commercially important crops worldwide and still the major food source in many developing countries. In order to accelerate the marker-assisted selection for sweet potato breeding, development and optimal use of easy-to-use and sequence-specific genetic markers, are required. Here, we analyzed 200 pairs of the random amplified polymorphic DNA (RAPD) markers to uncover the polymorphisms in sweet potato and subsequently developed 13 pairs of sequence-characterized amplified region (SCAR) markers. In contrast to the random amplification of RAPD markers, the newly-developed SCAR markers revealed lower ratio of polymorphisms, but showed higher repeatability, user-friendly and were sequence-specific. Furthermore, we employed these markers to exploit the genetic diversity of 27 Korean sweet potato cultivars, which could be distinguished and grouped. Overall, these results show that the newly-developed and sequence-specific SCAR markers could be used for marker-assisted selection (MAS) for breeding for elite sweet potato varieties in South Korea.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalPlant OMICS
Volume8
Issue number3
StatePublished - 2015

Keywords

  • Genetic diversity
  • Molecular markers
  • RAPD
  • SCAR
  • Sweet potato

Fingerprint

Dive into the research topics of 'Direct sequencing of RAPD products provides a set of SCAR markers for discrimination of sweet potato cultivars'. Together they form a unique fingerprint.

Cite this