Abstract
Sweet potato (Ipomoea batatas L. Lam) is one of the most commercially important crops worldwide and still the major food source in many developing countries. In order to accelerate the marker-assisted selection for sweet potato breeding, development and optimal use of easy-to-use and sequence-specific genetic markers, are required. Here, we analyzed 200 pairs of the random amplified polymorphic DNA (RAPD) markers to uncover the polymorphisms in sweet potato and subsequently developed 13 pairs of sequence-characterized amplified region (SCAR) markers. In contrast to the random amplification of RAPD markers, the newly-developed SCAR markers revealed lower ratio of polymorphisms, but showed higher repeatability, user-friendly and were sequence-specific. Furthermore, we employed these markers to exploit the genetic diversity of 27 Korean sweet potato cultivars, which could be distinguished and grouped. Overall, these results show that the newly-developed and sequence-specific SCAR markers could be used for marker-assisted selection (MAS) for breeding for elite sweet potato varieties in South Korea.
Original language | English |
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Pages (from-to) | 195-200 |
Number of pages | 6 |
Journal | Plant OMICS |
Volume | 8 |
Issue number | 3 |
State | Published - 2015 |
Keywords
- Genetic diversity
- Molecular markers
- RAPD
- SCAR
- Sweet potato