Effects of cadmium chloride and nonylphenol on the expression of StAR-related lipid transfer domain containing protein (START1) gene in aquatic midge, Chironomus riparius

Prakash M.Gopalakrishnan Nair, Jinhee Choi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We identified and characterized a partial cDNA of StAR-related lipid transfer domain containing protein gene from Chironomus riparius (CrSTART1) having homology with human MLN64 and Drosophila melanogaster START1 (DmSTART1) and evaluated the effects of cadmium chloride (Cd) and nonylphenol (NP) on its expression. Pfam analysis identified the presence of two StAR-related lipid transfer (START) domains in CrSTART1 having several conserved amino acid residues, characteristic of the MLN64 and DmSTART1. The mRNA expression of CrSTART1 was observed in all developmental stages. The modulation in the mRNA expression of CrSTART1 was investigated after exposure to different concentrations Cd (0, 2, 10, and 20 mg/L) and NP (0, 10, 50, and 100 μg/L) for different time intervals in fourth instar larvae of C. riparius. Significant downregulation of CrSTART1 mRNA was observed after exposure to 2, 10 and 20 mg/L of Cd for 24, 48 and 72 h. Significant upregulation of CrSTART1 was observed after exposure to 10 and 50 μg/L of NP for 24, and 48 h period. At 100 μg/L of NP significant upregulation of CrSTART1 was observed after 12 h and downregulated after 24, 48 and 72 h.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Volume155
Issue number2
DOIs
StatePublished - Mar 2012

Keywords

  • C. riparius
  • Cadmium chloride
  • Gene expression
  • Nonylphenol
  • START1

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