Effects of physical (hypoxia, hyperoxia) and chemical (potassium dichromate, fenitrothion) stress on antioxidant enzyme activities in Chironomus riparius Mg. (Diptera, Chironomidae) larvae: Potential biomarkers

Jinhee Choi, Hélène Roche, Thierry Caquet

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75 Scopus citations

Abstract

Effects of physical (hypoxia, hyperoxia) and chemical (potassium dichromate, fenitrothion) stress on antioxidant enzyme activities and hemoglobin content in Chironomus riparius Mg. (Diptera, Chironomidae) larvae were evaluated under laboratory conditions in order to identify pertinent biomarkers of stress. Hypoxia and hyperoxia caused an increase in Cu,Zn- superoxide dismutase (SOD) and Mn-SOD activities and a simultaneous decrease in total peroxidase (Px) and glutathione peroxidase (GSHPx) activities. A parallel increase in hemoglobin concentration in the hemolymph was observed. The exposure to sublethal concentrations of both potassium dichromate and fenitrothion led to an increase in Cu,Zn-SOD and Mn-SOD activities and to a decrease in GSH-Px activity. Activation of catalase (CAT) was observed in the larvae exposed to high fenitrothion concentration (μg/L). The response of SODs was rapid and sensitive to low chemical concentrations, but changes in CAT, Px, and GSH-Px were more specific and less sensitive.

Original languageEnglish
Pages (from-to)495-500
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Volume19
Issue number2
DOIs
StatePublished - 2000

Keywords

  • Antioxidant enzymes Biomarkers of stress
  • Chironomus riparius
  • Fenitrothion
  • Potassium dichromate

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