Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation

Hyunji Kim; Dajeong Kim; Sunghyun Moon; Jong Bum Lee

doi:10.1039/d4nr01770f

Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation

Hyunji Kim, Dajeong Kim, Sunghyun Moon, Jong Bum Lee

Department of Chemical Engineering

University of Seoul

Research output: Contribution to journal › Article › peer-review

Abstract

The COVID-19 pandemic heightened interest in circular RNA (C-RNA) for RNA therapeutics, offering advantages over linear mRNAs. Circular mRNA facilitates uncapped molecule development, and C-RNAs ensure stability in RNA interference therapeutics. The synthesis method, RNA ligation, is employed in C-RNA-based therapeutics. Stable DNA-RNA hybrid constructs enable efficient RNA ligase-based circularization.

Original language	English
Pages (from-to)	15529-15532
Number of pages	4
Journal	Nanoscale
Volume	16
Issue number	33
DOIs	https://doi.org/10.1039/d4nr01770f
State	Published - 5 Aug 2024

Access to Document

10.1039/d4nr01770f

Cite this

@article{f4de2c678f094cb7a7f3acc5d520b5b7,

title = "Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation",

abstract = "The COVID-19 pandemic heightened interest in circular RNA (C-RNA) for RNA therapeutics, offering advantages over linear mRNAs. Circular mRNA facilitates uncapped molecule development, and C-RNAs ensure stability in RNA interference therapeutics. The synthesis method, RNA ligation, is employed in C-RNA-based therapeutics. Stable DNA-RNA hybrid constructs enable efficient RNA ligase-based circularization.",

author = "Hyunji Kim and Dajeong Kim and Sunghyun Moon and Lee, {Jong Bum}",

note = "Publisher Copyright: {\textcopyright} 2024 The Royal Society of Chemistry.",

year = "2024",

month = aug,

day = "5",

doi = "10.1039/d4nr01770f",

language = "English",

volume = "16",

pages = "15529--15532",

journal = "Nanoscale",

issn = "2040-3364",

publisher = "Royal Society of Chemistry",

number = "33",

}

TY - JOUR

T1 - Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation

AU - Kim, Hyunji

AU - Kim, Dajeong

AU - Moon, Sunghyun

AU - Lee, Jong Bum

PY - 2024/8/5

Y1 - 2024/8/5

N2 - The COVID-19 pandemic heightened interest in circular RNA (C-RNA) for RNA therapeutics, offering advantages over linear mRNAs. Circular mRNA facilitates uncapped molecule development, and C-RNAs ensure stability in RNA interference therapeutics. The synthesis method, RNA ligation, is employed in C-RNA-based therapeutics. Stable DNA-RNA hybrid constructs enable efficient RNA ligase-based circularization.

AB - The COVID-19 pandemic heightened interest in circular RNA (C-RNA) for RNA therapeutics, offering advantages over linear mRNAs. Circular mRNA facilitates uncapped molecule development, and C-RNAs ensure stability in RNA interference therapeutics. The synthesis method, RNA ligation, is employed in C-RNA-based therapeutics. Stable DNA-RNA hybrid constructs enable efficient RNA ligase-based circularization.

UR - http://www.scopus.com/inward/record.url?scp=85200719064&partnerID=8YFLogxK

U2 - 10.1039/d4nr01770f

DO - 10.1039/d4nr01770f

M3 - Article

C2 - 39102212

AN - SCOPUS:85200719064

SN - 2040-3364

VL - 16

SP - 15529

EP - 15532

JO - Nanoscale

JF - Nanoscale

IS - 33

ER -

Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation

Abstract

Access to Document

Other files and links

Fingerprint

Cite this