Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation

Hyunji Kim; Dajeong Kim; Sunghyun Moon; Jong Bum Lee

doi:10.1039/d4nr01770f

Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation

Hyunji Kim, Dajeong Kim, Sunghyun Moon, Jong Bum Lee

Department of Chemical Engineering

University of Seoul

Research output: Contribution to journal › Article › peer-review

Abstract

The COVID-19 pandemic heightened interest in circular RNA (C-RNA) for RNA therapeutics, offering advantages over linear mRNAs. Circular mRNA facilitates uncapped molecule development, and C-RNAs ensure stability in RNA interference therapeutics. The synthesis method, RNA ligation, is employed in C-RNA-based therapeutics. Stable DNA-RNA hybrid constructs enable efficient RNA ligase-based circularization.

Original language	English
Pages (from-to)	15529-15532
Number of pages	4
Journal	Nanoscale
Volume	16
Issue number	33
DOIs	https://doi.org/10.1039/d4nr01770f
State	Published - 5 Aug 2024

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title = "Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation",

abstract = "The COVID-19 pandemic heightened interest in circular RNA (C-RNA) for RNA therapeutics, offering advantages over linear mRNAs. Circular mRNA facilitates uncapped molecule development, and C-RNAs ensure stability in RNA interference therapeutics. The synthesis method, RNA ligation, is employed in C-RNA-based therapeutics. Stable DNA-RNA hybrid constructs enable efficient RNA ligase-based circularization.",

author = "Hyunji Kim and Dajeong Kim and Sunghyun Moon and Lee, \{Jong Bum\}",

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Y1 - 2024/8/5

N2 - The COVID-19 pandemic heightened interest in circular RNA (C-RNA) for RNA therapeutics, offering advantages over linear mRNAs. Circular mRNA facilitates uncapped molecule development, and C-RNAs ensure stability in RNA interference therapeutics. The synthesis method, RNA ligation, is employed in C-RNA-based therapeutics. Stable DNA-RNA hybrid constructs enable efficient RNA ligase-based circularization.

AB - The COVID-19 pandemic heightened interest in circular RNA (C-RNA) for RNA therapeutics, offering advantages over linear mRNAs. Circular mRNA facilitates uncapped molecule development, and C-RNAs ensure stability in RNA interference therapeutics. The synthesis method, RNA ligation, is employed in C-RNA-based therapeutics. Stable DNA-RNA hybrid constructs enable efficient RNA ligase-based circularization.

UR - https://www.scopus.com/pages/publications/85200719064

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DO - 10.1039/d4nr01770f

M3 - Article

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JO - Nanoscale

JF - Nanoscale

IS - 33

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Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation

Abstract

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