Endoplasmic reticulum stress mediated apoptosis via jnk in mwcnt-exposed in vitro systems: Size, surface functionalization and cell type specificity

Nivedita Chatterjee, Jinhee Choi

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The aim of the present study was to evaluate the underlying mechanism of multi-walled carbon nanotubes (MWCNT) induced cellular response and their potential cross-talk, specifically, between endoplasmic reticulum (ER) stress, MAPK activation and apoptosis and how these nano-bio interactions depend on the physico-chemical properties of MWCNT. For this purpose, human bronchial epithelial (Beas2B) and human hepatoma (HepG2) cell lines, were exposed to five kinds of MWCNTs which dif-fer in functionalization and aspect ratios. Tissue-specific sensitivity was evident for calcium homeostasis, ER-stress response, MAPK activation and apoptosis, which further depended on surface functionalization as well as aspect ratios of MWCNT. By applying specific pharmaceutical inhibitors, relevant biomarkers gene and proteins expressions, we found that possibly MWCNT induce activation of IRE1α-XPB1 path-way-mediated ER-stress response, which in turn trigger apoptosis through JNK activation in both type of cells but with variable intensity. The information presented here would have relevance in better understanding of MWCNT toxicity and their safer applications.

Original languageEnglish
Pages (from-to)305-317
Number of pages13
JournalJournal of Toxicological Sciences
Volume45
Issue number6
DOIs
StatePublished - 2020

Keywords

  • Apoptosis
  • Calcium homeostasiEndoplasmic reticulum stress
  • In vitro cell type specificity
  • MAPK activation
  • MWCNTs

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