Enhanced production of biosurfactants through genetic engineering of Pseudozyma sp. SY16

Quynh Giao Tran, Ae Jin Ryu, Yong Jun Choi, Ki Jun Jeong, Hee Sik Kim, Yong Jae Lee

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Mannosylerythritol lipids (MELs) are natural glycolipids that possess biosurfactant properties and are abundantly produced by Pseudozyma sp. Due to their specific characteristics, such as biodegradability and low toxicity, MELs have attracted significant interest as an alternative to petroleum-based surfactants in medical and cosmetic fields. The present study describes a novel expression system and optimal transformation conditions of Pseudozyma sp. SY16 for producing MELs. The hygromycin resistance gene under the control of Deinococcus radiodurans-derived Kat1 promoter was used as selection marker and the superfolder green fluorescent protein under the control of the yeast glyceraldehyde-3-phosphate dehydrogenase promoter was used for confirming successful expression. Using this expression system, several transformants overexpressing genes related to MEL production, including emt1, mmf1, and mat1, were generated. Among them, MMF1-2 strain exhibited an MEL yield of 27.9 g/L, which was 31.6% higher than that of the wild-type strain. Altogether, this study demonstrates that engineered yeast strains hold potential for large-scale production of MELs.

Original languageEnglish
Pages (from-to)997-1003
Number of pages7
JournalKorean Journal of Chemical Engineering
Volume39
Issue number4
DOIs
StatePublished - Apr 2022

Keywords

  • Biosurfactant
  • MEL
  • Pseudozyma sp
  • Transformation
  • Yeast

Fingerprint

Dive into the research topics of 'Enhanced production of biosurfactants through genetic engineering of Pseudozyma sp. SY16'. Together they form a unique fingerprint.

Cite this