Ginseng gintonin enhances hyaluronic acid and collagen release from human dermal fibroblasts through lysophosphatidic acid receptor interaction

Rami Lee, Na Eun Lee, Hongik Hwang, Hyewhon Rhim, Ik Hyun Cho, Seung Yeol Nah

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Gintonin is a newly discovered component of ginseng and acts as a ligand for G protein-coupled lysophosphatidic acid (LPA) receptors. It is currently unclear whether gintonin has skin-related effects. Here, we examined the effects of a gintonin-enriched fraction (GEF) on [Ca2+]i transient induction in human dermal fibroblasts (HDFs). We found that GEF treatment transiently induced [Ca2+]i in a dose-dependent manner. GEF also increased cell viability and proliferation, which could be blocked by Ki16425, an LPA1/3 receptor antagonist, or 1,2-Bis(2-aminophenoxy)ethane-N,N,N0,N0-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), a calcium chelator. We further found that GEF stimulated hyaluronic acid (HA) release fromHDFs in a dose- and time-dependentmanner,which could be attenuated by Ki16425, U73122, a phospholipase C inhibitor, 2-Aminoethoxydiphenyl borate (2-APB), an IP3 receptor antagonist, and BAPTA-AM.Moreover, we found that GEF increased HA synthase 1 (HAS1) expression in a time-dependentmanner. We also found that GEF stimulates collagen release and the expression of collagen 1, 3, and 7 synthases in a time-dependent manner. GEF-mediated collagen synthesis could be blocked by Ki16425, U73122, 2-APB, and BAPTA-AM. GEF treatment also increased the mRNA levels of LPA1-6 receptor subtypes at 8 h and increased the protein levels of LPA1-6 receptor subtypes at 8 h. Overall, these results indicate that the GEF-mediated transient induction of [Ca2+]i is coupled to HA and collagen release fromHDFs via LPA receptor regulations. We can, thus, conclude that GEFmight exert a beneficial effect on human skin physiology via LPA receptors.

Original languageEnglish
Article number4438
JournalMolecules
Volume24
Issue number24
DOIs
StatePublished - 4 Dec 2019

Keywords

  • Collagen
  • Ginseng
  • Gintonin
  • Human dermal fibroblast
  • Human skin
  • Hyaluronic acid

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