High-Yield Production of Lycopene from Corn Steep Liquor and Glycerol Using the Metabolically Engineered Deinococcus radiodurans R1 Strain

Chang Keun Kang, Sun Wook Jeong, Jung Eun Yang, Yong Jun Choi

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Developing a highly efficient and ecofriendly system to produce desired products from waste can be considered important to a sustainable society. Here, we report for the first time high-yield production of lycopene through metabolically engineering an extremophilic microorganism, Deinococcus radiodurans R1, from corn steep liquor (CSL) and glycerol. First, the crtLm gene-encoding lycopene cyclase was deleted to prevent the conversion of lycopene to γ-carotene. Then, the crtB gene-encoding phytoene synthase and the dxs gene-encoding 1-deoxy-d-xylulose 5-phosphate synthase were overexpressed to increase carbon flux toward lycopene. The engineered ΔcrtLm/crtB+dxs+ D. radiodurans R1 could produce 273.8 mg/L [80.7 mg/g dry cell weight (DCW)] and 373.5 mg/L (108.0 mg/g DCW) of lycopene from 10 g/L of glucose with 5 g/L of yeast extract and 9.9 g/L of glucose with 20 g/L of CSL, respectively. Moreover, the lycopene titer and content were increased by 26% (470.6 mg/L) and 28% (138.2 mg/g DCW), respectively, when the carbon source was changed to glycerol. Finally, fed-batch fermentation of the final engineered strain allowed the production of 722.2 mg/L (203.5 mg/g DCW) of lycopene with a yield and productivity of 20.3 mg/g glycerol and 6.0 mg/L/h, respectively, from 25 g/L of CSL and 35.7 g/L of glycerol.

Original languageEnglish
Pages (from-to)5147-5153
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume68
Issue number18
DOIs
StatePublished - 6 May 2020

Keywords

  • Deinococcus radiodurans R1
  • corn steep liquor
  • glycerol
  • lycopene
  • metabolic engineering

Fingerprint

Dive into the research topics of 'High-Yield Production of Lycopene from Corn Steep Liquor and Glycerol Using the Metabolically Engineered Deinococcus radiodurans R1 Strain'. Together they form a unique fingerprint.

Cite this