Highly fluorescent and water soluble turn-on type diarylethene for super-resolution bioimaging over a broad pH range

Sang Hoon Um; Hyung Jun Kim; Dojin Kim; Ji Eon Kwon; Jong Woo Lee; Doyk Hwang; Seong Keun Kim; Soo Young Park

doi:10.1016/j.dyepig.2018.05.014

Highly fluorescent and water soluble turn-on type diarylethene for super-resolution bioimaging over a broad pH range

Sang Hoon Um, Hyung Jun Kim, Dojin Kim, Ji Eon Kwon, Jong Woo Lee, Doyk Hwang, Seong Keun Kim, Soo Young Park

Department of Applied Chemistry

Seoul National University

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We report a water-soluble turn-on type diarylethene SO3-BPDBTEO that reversibly switches its fluorescence on and off upon light stimuli. We note that the two sodium sulfonate groups enable SO3-BPDBTEO to exhibit excellent photo-switching properties in aqueous solution over a wide range of pH between 3 and 10. We demonstrated that SO3-BPDBTEO could be applied to conventional fluorescence imaging of mammalian cell lines such as HeLa cell at various pH conditions with low cytotoxicity. We also examine its feasibility for super-resolution fluorescence imaging as a photoswitching probe.

Original language	English
Pages (from-to)	36-41
Number of pages	6
Journal	Dyes and Pigments
Volume	158
DOIs	https://doi.org/10.1016/j.dyepig.2018.05.014
State	Published - Nov 2018

Keywords

Bioimaging
Broad pH range
Fluorescence
Photochromism
Super-resolution

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10.1016/j.dyepig.2018.05.014

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title = "Highly fluorescent and water soluble turn-on type diarylethene for super-resolution bioimaging over a broad pH range",

abstract = "We report a water-soluble turn-on type diarylethene SO3-BPDBTEO that reversibly switches its fluorescence on and off upon light stimuli. We note that the two sodium sulfonate groups enable SO3-BPDBTEO to exhibit excellent photo-switching properties in aqueous solution over a wide range of pH between 3 and 10. We demonstrated that SO3-BPDBTEO could be applied to conventional fluorescence imaging of mammalian cell lines such as HeLa cell at various pH conditions with low cytotoxicity. We also examine its feasibility for super-resolution fluorescence imaging as a photoswitching probe.",

keywords = "Bioimaging, Broad pH range, Fluorescence, Photochromism, Super-resolution",

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doi = "10.1016/j.dyepig.2018.05.014",

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T1 - Highly fluorescent and water soluble turn-on type diarylethene for super-resolution bioimaging over a broad pH range

AU - Um, Sang Hoon

AU - Kim, Hyung Jun

AU - Kim, Dojin

AU - Kwon, Ji Eon

AU - Lee, Jong Woo

AU - Hwang, Doyk

AU - Kim, Seong Keun

AU - Park, Soo Young

PY - 2018/11

Y1 - 2018/11

N2 - We report a water-soluble turn-on type diarylethene SO3-BPDBTEO that reversibly switches its fluorescence on and off upon light stimuli. We note that the two sodium sulfonate groups enable SO3-BPDBTEO to exhibit excellent photo-switching properties in aqueous solution over a wide range of pH between 3 and 10. We demonstrated that SO3-BPDBTEO could be applied to conventional fluorescence imaging of mammalian cell lines such as HeLa cell at various pH conditions with low cytotoxicity. We also examine its feasibility for super-resolution fluorescence imaging as a photoswitching probe.

AB - We report a water-soluble turn-on type diarylethene SO3-BPDBTEO that reversibly switches its fluorescence on and off upon light stimuli. We note that the two sodium sulfonate groups enable SO3-BPDBTEO to exhibit excellent photo-switching properties in aqueous solution over a wide range of pH between 3 and 10. We demonstrated that SO3-BPDBTEO could be applied to conventional fluorescence imaging of mammalian cell lines such as HeLa cell at various pH conditions with low cytotoxicity. We also examine its feasibility for super-resolution fluorescence imaging as a photoswitching probe.

KW - Bioimaging

KW - Broad pH range

KW - Fluorescence

KW - Photochromism

KW - Super-resolution

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DO - 10.1016/j.dyepig.2018.05.014

M3 - Article

AN - SCOPUS:85047090915

SN - 0143-7208

VL - 158

SP - 36

EP - 41

JO - Dyes and Pigments

JF - Dyes and Pigments

ER -

Highly fluorescent and water soluble turn-on type diarylethene for super-resolution bioimaging over a broad pH range

Abstract

Keywords

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