Hydrogen peroxide negatively modulates Wnt signaling through downregulation of β-catenin

Soon Young Shin; Chang Gun Kim; Eek Hoon Jho; Myung Sun Rho; Yong Sik Kim; Young Ho Kim; Young Han Lee

doi:10.1016/j.canlet.2004.03.003

Hydrogen peroxide negatively modulates Wnt signaling through downregulation of β-catenin

Soon Young Shin, Chang Gun Kim, Eek Hoon Jho, Myung Sun Rho, Yong Sik Kim, Young Ho Kim, Young Han Lee

Department of Life Science

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

The Wnt signal transduction pathway plays an important role in organogenesis and carcinogenesis. In an effort to better understand the action of oxidative stress-induced cellular signaling, we investigate the effect of exogenous H ₂O ₂ on the Wnt signal pathway. H ₂O ₂ decreases the amount of nuclear β-catenin and Tcf/Lef-dependent transcription. Overexpression of Dvl-1 abrogated H ₂O ₂-induced downregulation of β-catenin. Pretreatment with LiCl or Wnt-3a conditioned medium completely inhibited H ₂O ₂-induced release of mitochondrial cytochrome c and DNA fragmentation. These results suggest that H ₂O ₂ negatively modulates the Wnt signal pathway through downregulation of β-catenin.

Original language	English
Pages (from-to)	225-231
Number of pages	7
Journal	Cancer Letters
Volume	212
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2004.03.003
State	Published - 30 Aug 2004

Keywords

Cytochrome c
DNA fragmentation
Disheveled
Hydrogen peroxide
Tcf/Lef
Wnt-3a
β-Catenin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2004.03.003

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title = "Hydrogen peroxide negatively modulates Wnt signaling through downregulation of β-catenin",

abstract = "The Wnt signal transduction pathway plays an important role in organogenesis and carcinogenesis. In an effort to better understand the action of oxidative stress-induced cellular signaling, we investigate the effect of exogenous H 2O 2 on the Wnt signal pathway. H 2O 2 decreases the amount of nuclear β-catenin and Tcf/Lef-dependent transcription. Overexpression of Dvl-1 abrogated H 2O 2-induced downregulation of β-catenin. Pretreatment with LiCl or Wnt-3a conditioned medium completely inhibited H 2O 2-induced release of mitochondrial cytochrome c and DNA fragmentation. These results suggest that H 2O 2 negatively modulates the Wnt signal pathway through downregulation of β-catenin.",

keywords = "Cytochrome c, DNA fragmentation, Disheveled, Hydrogen peroxide, Tcf/Lef, Wnt-3a, β-Catenin",

author = "Shin, {Soon Young} and Kim, {Chang Gun} and Jho, {Eek Hoon} and Rho, {Myung Sun} and Kim, {Yong Sik} and Kim, {Young Ho} and Lee, {Young Han}",

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doi = "10.1016/j.canlet.2004.03.003",

language = "English",

volume = "212",

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journal = "Cancer Letters",

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T1 - Hydrogen peroxide negatively modulates Wnt signaling through downregulation of β-catenin

AU - Shin, Soon Young

AU - Kim, Chang Gun

AU - Jho, Eek Hoon

AU - Rho, Myung Sun

AU - Kim, Yong Sik

AU - Kim, Young Ho

AU - Lee, Young Han

PY - 2004/8/30

Y1 - 2004/8/30

N2 - The Wnt signal transduction pathway plays an important role in organogenesis and carcinogenesis. In an effort to better understand the action of oxidative stress-induced cellular signaling, we investigate the effect of exogenous H 2O 2 on the Wnt signal pathway. H 2O 2 decreases the amount of nuclear β-catenin and Tcf/Lef-dependent transcription. Overexpression of Dvl-1 abrogated H 2O 2-induced downregulation of β-catenin. Pretreatment with LiCl or Wnt-3a conditioned medium completely inhibited H 2O 2-induced release of mitochondrial cytochrome c and DNA fragmentation. These results suggest that H 2O 2 negatively modulates the Wnt signal pathway through downregulation of β-catenin.

AB - The Wnt signal transduction pathway plays an important role in organogenesis and carcinogenesis. In an effort to better understand the action of oxidative stress-induced cellular signaling, we investigate the effect of exogenous H 2O 2 on the Wnt signal pathway. H 2O 2 decreases the amount of nuclear β-catenin and Tcf/Lef-dependent transcription. Overexpression of Dvl-1 abrogated H 2O 2-induced downregulation of β-catenin. Pretreatment with LiCl or Wnt-3a conditioned medium completely inhibited H 2O 2-induced release of mitochondrial cytochrome c and DNA fragmentation. These results suggest that H 2O 2 negatively modulates the Wnt signal pathway through downregulation of β-catenin.

KW - Cytochrome c

KW - DNA fragmentation

KW - Disheveled

KW - Hydrogen peroxide

KW - Tcf/Lef

KW - Wnt-3a

KW - β-Catenin

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U2 - 10.1016/j.canlet.2004.03.003

DO - 10.1016/j.canlet.2004.03.003

M3 - Article

C2 - 15279902

AN - SCOPUS:3242883359

SN - 0304-3835

VL - 212

SP - 225

EP - 231

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Hydrogen peroxide negatively modulates Wnt signaling through downregulation of β-catenin

Abstract

Keywords

UN SDGs

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