Identification of ovarian enhancer-binding factors which bind to ovarian enhancer 1 of the Drosophila genes yp1 and yp2

Yun Doo Chung, Hyeok Choon Kwon, Ki Wha Chung, Se Jae Kim, Kyungjin Kim, Chung Choo Lee

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

It has been reported that three different DNA regions - the fat body enhancer and ovarian enhancers 1 and 2 - direct the tissue-specific expression of yp1 and yp2 in Drosophila melanogaster. In the present study, we identified ovarian enhancer 1-specific binding proteins. Electrophoretic mobility shift assay revealed that these proteins are present in the adult ovary, but not in adult testis or fat body. Southwestern blot analysis showed that about 130 kDa and 40 kDa proteins, designated OEF1 and OEF2, respectively, from ovarian nuclear or crude extracts bind specifically to the ovarian enhancer 1. The two proteins were partially purified by streptavidin/agarose-DNA affinity chromatography, and their binding activity was confirmed by electrophoretic mobility shift assay. These ovarian enhancer factors may play an important role in the regulation of transcription of yp1 and yp2 in the ovary.

Original languageEnglish
Pages (from-to)347-351
Number of pages5
JournalMolecular and General Genetics
Volume251
Issue number3
DOIs
StatePublished - 1996

Keywords

  • Drosophila melanogaster
  • Enhancer-binding factors
  • Ovarian enhancer 1
  • Yolk protein

Fingerprint

Dive into the research topics of 'Identification of ovarian enhancer-binding factors which bind to ovarian enhancer 1 of the Drosophila genes yp1 and yp2'. Together they form a unique fingerprint.

Cite this