Identification of ptpro as a novel target gene of Wnt signaling and its potential role as a receptor for Wnt

Minseong Kim; Hanjun Kim; Eek hoon Jho

doi:10.1016/j.febslet.2010.08.034

Identification of ptpro as a novel target gene of Wnt signaling and its potential role as a receptor for Wnt

Minseong Kim, Hanjun Kim, Eek hoon Jho

Department of Life Science

University of Seoul

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Wnt/β-catenin signaling plays critical roles in embryonic development and tissue homeostasis in adults by controlling the expression of target genes. We found that expression of ptpro, which encodes a protein tyrosine phosphatase receptor type O (PTPRO), was induced by Wnt/β-catenin signaling in a T cell factor/lymphoid enhancer factor dependent manner. Biochemical assays found that PTPRO interacted with Wnt via its extracellular domain. In addition, ectopic expression of this extracellular domain inhibited Wnt-mediated reporter activity. These results suggest that ptpro is a target gene of Wnt/β-catenin signaling and that PTPRO may function as a novel receptor for Wnt. Structured summary: MINT- 7992076: Ptpro (uniprotkb:. Q7TSY7) physically interacts (MI:. 0915) with Wnt3a (uniprotkb:. P27467) by anti tag coimmunoprecipitation (MI:. 0007). MINT- 7992094: Ptpro (uniprotkb:. Q7TSY7) physically interacts (MI:. 0915) with Wnt-3a (uniprotkb:. P27467) by cross-linking study (MI:. 0030).

Original language	English
Pages (from-to)	3923-3928
Number of pages	6
Journal	FEBS Letters
Volume	584
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2010.08.034
State	Published - Sep 2010

Keywords

Protein tyrosine phosphatase receptor type O
T cell factor/lymphoid enhancer factor
Wnt
Wnt receptor
β-Catenin

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10.1016/j.febslet.2010.08.034

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title = "Identification of ptpro as a novel target gene of Wnt signaling and its potential role as a receptor for Wnt",

abstract = "Wnt/β-catenin signaling plays critical roles in embryonic development and tissue homeostasis in adults by controlling the expression of target genes. We found that expression of ptpro, which encodes a protein tyrosine phosphatase receptor type O (PTPRO), was induced by Wnt/β-catenin signaling in a T cell factor/lymphoid enhancer factor dependent manner. Biochemical assays found that PTPRO interacted with Wnt via its extracellular domain. In addition, ectopic expression of this extracellular domain inhibited Wnt-mediated reporter activity. These results suggest that ptpro is a target gene of Wnt/β-catenin signaling and that PTPRO may function as a novel receptor for Wnt. Structured summary: MINT- 7992076: Ptpro (uniprotkb:. Q7TSY7) physically interacts (MI:. 0915) with Wnt3a (uniprotkb:. P27467) by anti tag coimmunoprecipitation (MI:. 0007). MINT- 7992094: Ptpro (uniprotkb:. Q7TSY7) physically interacts (MI:. 0915) with Wnt-3a (uniprotkb:. P27467) by cross-linking study (MI:. 0030).",

keywords = "Protein tyrosine phosphatase receptor type O, T cell factor/lymphoid enhancer factor, Wnt, Wnt receptor, β-Catenin",

author = "Minseong Kim and Hanjun Kim and Jho, {Eek hoon}",

year = "2010",

month = sep,

doi = "10.1016/j.febslet.2010.08.034",

language = "English",

volume = "584",

pages = "3923--3928",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "18",

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TY - JOUR

T1 - Identification of ptpro as a novel target gene of Wnt signaling and its potential role as a receptor for Wnt

AU - Kim, Minseong

AU - Kim, Hanjun

AU - Jho, Eek hoon

PY - 2010/9

Y1 - 2010/9

N2 - Wnt/β-catenin signaling plays critical roles in embryonic development and tissue homeostasis in adults by controlling the expression of target genes. We found that expression of ptpro, which encodes a protein tyrosine phosphatase receptor type O (PTPRO), was induced by Wnt/β-catenin signaling in a T cell factor/lymphoid enhancer factor dependent manner. Biochemical assays found that PTPRO interacted with Wnt via its extracellular domain. In addition, ectopic expression of this extracellular domain inhibited Wnt-mediated reporter activity. These results suggest that ptpro is a target gene of Wnt/β-catenin signaling and that PTPRO may function as a novel receptor for Wnt. Structured summary: MINT- 7992076: Ptpro (uniprotkb:. Q7TSY7) physically interacts (MI:. 0915) with Wnt3a (uniprotkb:. P27467) by anti tag coimmunoprecipitation (MI:. 0007). MINT- 7992094: Ptpro (uniprotkb:. Q7TSY7) physically interacts (MI:. 0915) with Wnt-3a (uniprotkb:. P27467) by cross-linking study (MI:. 0030).

AB - Wnt/β-catenin signaling plays critical roles in embryonic development and tissue homeostasis in adults by controlling the expression of target genes. We found that expression of ptpro, which encodes a protein tyrosine phosphatase receptor type O (PTPRO), was induced by Wnt/β-catenin signaling in a T cell factor/lymphoid enhancer factor dependent manner. Biochemical assays found that PTPRO interacted with Wnt via its extracellular domain. In addition, ectopic expression of this extracellular domain inhibited Wnt-mediated reporter activity. These results suggest that ptpro is a target gene of Wnt/β-catenin signaling and that PTPRO may function as a novel receptor for Wnt. Structured summary: MINT- 7992076: Ptpro (uniprotkb:. Q7TSY7) physically interacts (MI:. 0915) with Wnt3a (uniprotkb:. P27467) by anti tag coimmunoprecipitation (MI:. 0007). MINT- 7992094: Ptpro (uniprotkb:. Q7TSY7) physically interacts (MI:. 0915) with Wnt-3a (uniprotkb:. P27467) by cross-linking study (MI:. 0030).

KW - Protein tyrosine phosphatase receptor type O

KW - T cell factor/lymphoid enhancer factor

KW - Wnt

KW - Wnt receptor

KW - β-Catenin

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DO - 10.1016/j.febslet.2010.08.034

M3 - Article

C2 - 20804755

AN - SCOPUS:77956913115

SN - 0014-5793

VL - 584

SP - 3923

EP - 3928

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

Identification of ptpro as a novel target gene of Wnt signaling and its potential role as a receptor for Wnt

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Keywords

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