In situ extractive fermentation for the production of hexanoic acid from galactitol by Clostridium sp. BS-1

Byoung Seung Jeon, Chuloo Moon, Byung Chun Kim, Hyunook Kim, Youngsoon Um, Byoung In Sang

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Clostridium sp. BS-1 produces hexanoic acid as a metabolite using galactitol and enhanced hexanoic acid production was obtained by in situ extractive fermentation with Clostridium sp. BS-1 under an optimized medium composition. For medium optimization, five ingredients were selected as variables, and among them yeast extract, tryptone, and sodium butyrate were selected as significant variables according to a fractional factorial experimental design, a steepest ascent experimental design, and a Box-Behnken experimental design. The optimized medium had the following compositions in modified Clostridium acetobutyricum (mCAB) medium: 15.5gL-1 of yeast extract, 10.13gL-1 of tryptone, 0.04gL-1 of FeSO4·7H2O, 0.85gL-1 of sodium acetate, and 6.47gL-1 of sodium butyrate. The predicted concentration of hexanoic acid with the optimized medium was 6.98gL-1, and this was validated experimentally by producing 6.96gL-1 of hexanoic acid with Clostridium sp. BS-1 under the optimized conditions. In situ extractive fermentation for hexanoic acid removal was then applied in a batch culture system with the optimized medium and 10% (v/v) alamine 336 in oleyl alcohol as an extractive solvent. The pH of the culture in the extractive fermentation was maintained at 5.4-5.6 by an acid balance between production and retrieval by extraction. During a 16 day culture, the hexanoic acid concentration in the solvent increased to 32gL-1 while it was maintained in a range of 1-2gL-1 in the medium. The maximum rate of hexanoic acid production was 0.34gL-1h-1 in in situ extractive fermentation.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalEnzyme and Microbial Technology
Issue number3
StatePublished - 15 Aug 2013


  • Box-Behnken experimental design
  • Clostridium sp. BS-1
  • Fractional factorial experimental design
  • Galactitol
  • Hexanoic acid
  • In situ extractive fermentation


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