Inhibition of cysteine proteinases by carica papaya cystatin produced in escherichia coli

I. Song; M. Taylor; K. Baker; R. C. Bateman

doi:10.1016/0378-1119(95)00258-8

Inhibition of cysteine proteinases by carica papaya cystatin produced in escherichia coli

I. Song, M. Taylor, K. Baker, R. C. Bateman

Department of Life Science

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

A papaya cystatin (Cst)-encoding cDNA clone was isolated from a papaya leaf cDNA library and the active protein produced in Escherichia coli. The amino-acid sequence reveals a protein of 11262 Da with over 40% identity to other published plant Cst. Unique features of the papaya Cst include a single Cys residue, variation in the papain-binding region, and the first reported inhibition of papaya proteinase IV by a Cst.

Original language	English
Pages (from-to)	221-224
Number of pages	4
Journal	Gene
Volume	162
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(95)00258-8
State	Published - 11 Sep 1995

Keywords

Cst
ORF
PAGE
PPIV
Papain
SDS
UTR
[]
aa
amino acid(s)
base pair(s)
bp
caricain
chymopapain
cystatin(s)
denotes plasmid-carrier state
kb
kilobase(s) or 1000 bp
nt
nucleotide(s)
open reading frame
p-nitroanaline
pNA
papaya protease IV
phytocystatin
plant
polyacrylamide-gel electrophoresis
re-
recombinant
sodium dodecyl sulfate
untranslated region(s)

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10.1016/0378-1119(95)00258-8

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title = "Inhibition of cysteine proteinases by carica papaya cystatin produced in escherichia coli",

abstract = "A papaya cystatin (Cst)-encoding cDNA clone was isolated from a papaya leaf cDNA library and the active protein produced in Escherichia coli. The amino-acid sequence reveals a protein of 11262 Da with over 40% identity to other published plant Cst. Unique features of the papaya Cst include a single Cys residue, variation in the papain-binding region, and the first reported inhibition of papaya proteinase IV by a Cst.",

keywords = "Cst, ORF, PAGE, PPIV, Papain, SDS, UTR, [], aa, amino acid(s), base pair(s), bp, caricain, chymopapain, cystatin(s), denotes plasmid-carrier state, kb, kilobase(s) or 1000 bp, nt, nucleotide(s), open reading frame, p-nitroanaline, pNA, papaya protease IV, phytocystatin, plant, polyacrylamide-gel electrophoresis, re-, recombinant, sodium dodecyl sulfate, untranslated region(s)",

author = "I. Song and M. Taylor and K. Baker and Bateman, {R. C.}",

year = "1995",

month = sep,

day = "11",

doi = "10.1016/0378-1119(95)00258-8",

language = "English",

volume = "162",

pages = "221--224",

journal = "Gene",

issn = "0378-1119",

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number = "2",

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TY - JOUR

T1 - Inhibition of cysteine proteinases by carica papaya cystatin produced in escherichia coli

AU - Song, I.

AU - Taylor, M.

AU - Baker, K.

AU - Bateman, R. C.

PY - 1995/9/11

Y1 - 1995/9/11

N2 - A papaya cystatin (Cst)-encoding cDNA clone was isolated from a papaya leaf cDNA library and the active protein produced in Escherichia coli. The amino-acid sequence reveals a protein of 11262 Da with over 40% identity to other published plant Cst. Unique features of the papaya Cst include a single Cys residue, variation in the papain-binding region, and the first reported inhibition of papaya proteinase IV by a Cst.

AB - A papaya cystatin (Cst)-encoding cDNA clone was isolated from a papaya leaf cDNA library and the active protein produced in Escherichia coli. The amino-acid sequence reveals a protein of 11262 Da with over 40% identity to other published plant Cst. Unique features of the papaya Cst include a single Cys residue, variation in the papain-binding region, and the first reported inhibition of papaya proteinase IV by a Cst.

KW - Cst

KW - ORF

KW - PAGE

KW - PPIV

KW - Papain

KW - SDS

KW - UTR

KW - []

KW - aa

KW - amino acid(s)

KW - base pair(s)

KW - bp

KW - caricain

KW - chymopapain

KW - cystatin(s)

KW - denotes plasmid-carrier state

KW - kb

KW - kilobase(s) or 1000 bp

KW - nt

KW - nucleotide(s)

KW - open reading frame

KW - p-nitroanaline

KW - pNA

KW - papaya protease IV

KW - phytocystatin

KW - plant

KW - polyacrylamide-gel electrophoresis

KW - re-

KW - recombinant

KW - sodium dodecyl sulfate

KW - untranslated region(s)

UR - http://www.scopus.com/inward/record.url?scp=0029091413&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(95)00258-8

DO - 10.1016/0378-1119(95)00258-8

M3 - Article

C2 - 7557432

AN - SCOPUS:0029091413

SN - 0378-1119

VL - 162

SP - 221

EP - 224

JO - Gene

JF - Gene

IS - 2

ER -

Inhibition of cysteine proteinases by carica papaya cystatin produced in escherichia coli

Abstract

Keywords

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