Integrating fermentation of Chlamydomonas mexicana by oleaginous Lipomyces starkeyi and switchable ionic liquid extraction for enhanced biodiesel production

Gyeong Uk Kim, Geon Soo Ha, Mayur B. Kurade, Shouvik Saha, Moonis Ali Khan, Young Kwon Park, Woojin Chung, Soon Woong Chang, Krishna Kumar Yadav, Byong Hun Jeon

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The conversion of multi-bioconstituents of microalgal biomass such as carbohydrates and proteins to lipids can substantially reduce the downstream and overall process cost, as the bioprocessing can be aimed at single-desired biofuel product, e.g., biodiesel. The present study developed an integrated process to enhance the lipid yield through oleaginous yeast fermentation of microalgal biomass, and cost-effectively extract the lipids through permeable and switchable ionic liquid (IL). A 60 g/L of microalgal (Chlamydomonas mexicana) biomass was pretreated via microwave which showed 37% cell disruption efficiency. The pretreated biomass was fermented using an oleaginous yeast, L. starkeyi which effectively consumed the carbohydrates (92%) and proteins (91%) of C. mexicana and converted it to excessive lipids (188%) in comparison to the original microalgal lipids (12.6 g/L) before fermentation. The ionic liquid (IL), Dissopropanolamine-Im was used for high-thoughput disruption of the biomass and extraction of total lipids. It significantly improved cell disruption efficiency (95%) and lipid extraction efficiency (99%) than the conventional (Bligh & Dyer) lipid extraction method (87%) and previously reported methods using several ionic liquids (∼92%), and required a relatively shorter process time. More than 93% of IL could be recovored from the solution, and its recyclability was tested for >5 times where it could maintain an efficiency of >80% after five cycles. The integration of microalgal biomass and L. starkeyi mediated fermentation improved the overall lipid production (23.72 g/L) and yielded 2.2 times higher biodiesel than the conventional process. The outcomes of this study provides a economic and sustainable model to overcome the existing limitations of biodiesel production from microalgal biomass.

Original languageEnglish
Article number137285
JournalChemical Engineering Journal
Volume446
DOIs
StatePublished - 15 Oct 2022

Keywords

  • Biodiesel
  • Fermentation
  • Ionic liquid
  • Microalgal biomass
  • Oleaginous yeast

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