Investigation of förster resonance energy transfer (FRET) and competition of fluorescent dyes on DNA microparticles

Jieun Kim, Jae Sung Lee, Jong Bum Lee

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Fluorescent labeling is widely used to investigate the structural stability and changes to DNA nano- and microstructures. Despite this, the conventional method for observing DNA structures has several limitations in terms of cost-efficiency. This paper introduces a DNA spherical particle stained with DNA intercalating dyes (SYBR Green and SYTOX Orange) as tracers and reports the interaction between multiple dyes. The interference between the dyes was analyzed in terms of Förster resonance energy transfer (FRET) and competition. The changes in the fluorescence intensity by FRET were uniform, regardless of the sequence. The competition effect could occur when several dyes were added simultaneously. These properties are expected to help in the design of multicolor tracers in bioimaging and environmental applications.

Original languageEnglish
Pages (from-to)7738-7747
Number of pages10
JournalInternational Journal of Molecular Sciences
Volume16
Issue number4
DOIs
StatePublished - 8 Apr 2015

Keywords

  • Bioimaging
  • DNA microparticles
  • DNA technology
  • Förster resonance energy transfer (FRET)

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