TY - JOUR
T1 - Iron (III) oxide nanoparticles alleviate arsenic induced stunting in Vigna radiata
AU - Shabnam, Nisha
AU - Kim, Minsoo
AU - Kim, Hyunook
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/11/15
Y1 - 2019/11/15
N2 - Iron nanoparticles (NPs) are widely used for the removal of arsenic from water. In this study, we evaluated the interaction between arsenate (AsO4 3−) and Fe2O3-NPs on early seedling growth of Vigna radiata. Seedlings were raised in AsO4 3− and Fe2O3-NPs, alone and in combination. While Fe2O3-NPs slightly promoted seedling growth, AsO4 3− reduced seedling growth drastically. AsO4 3--induced decline in the seedling growth was recovered by Fe2O3-NPs. In contrast, equivalent concentrations of FeCl3, alone and together with AsO4 3−, inhibited seed germination completely. Lower arsenic content in seedlings raised in the presence of Fe2O3-NPs indicated that Fe2O3-NPs restricted arsenic uptake. Ability of Fe2O3-NPs to restrict the arsenic uptake of the seedlings was due to adsorption of AsO4 3−, as revealed by transmission and scanning electron microscopy. Non-toxic levels of iron in seedlings were due to restriction of Fe2O3-NPs to root-surface. AsO4 3− enhanced the ferric chelate reductase activity of root which was recovered by Fe2O3-NPs. The AsO4 3--induced oxidative stress, evident from high levels of proline, H2O2 and malondialdehyde, and lowered root oxidisability was ameliorated by Fe2O3-NPs. AsO4 3-induced enhancement in total antioxidant capacity, superoxide dismutase and catalase activity, and decline in guaiacol peroxidase activity were antagonized by Fe2O3-NPs. Our findings reveal that Fe2O3-NPs provide effective resistance/amelioration to arsenic toxicity by reducing arsenic availability to plants.
AB - Iron nanoparticles (NPs) are widely used for the removal of arsenic from water. In this study, we evaluated the interaction between arsenate (AsO4 3−) and Fe2O3-NPs on early seedling growth of Vigna radiata. Seedlings were raised in AsO4 3− and Fe2O3-NPs, alone and in combination. While Fe2O3-NPs slightly promoted seedling growth, AsO4 3− reduced seedling growth drastically. AsO4 3--induced decline in the seedling growth was recovered by Fe2O3-NPs. In contrast, equivalent concentrations of FeCl3, alone and together with AsO4 3−, inhibited seed germination completely. Lower arsenic content in seedlings raised in the presence of Fe2O3-NPs indicated that Fe2O3-NPs restricted arsenic uptake. Ability of Fe2O3-NPs to restrict the arsenic uptake of the seedlings was due to adsorption of AsO4 3−, as revealed by transmission and scanning electron microscopy. Non-toxic levels of iron in seedlings were due to restriction of Fe2O3-NPs to root-surface. AsO4 3− enhanced the ferric chelate reductase activity of root which was recovered by Fe2O3-NPs. The AsO4 3--induced oxidative stress, evident from high levels of proline, H2O2 and malondialdehyde, and lowered root oxidisability was ameliorated by Fe2O3-NPs. AsO4 3-induced enhancement in total antioxidant capacity, superoxide dismutase and catalase activity, and decline in guaiacol peroxidase activity were antagonized by Fe2O3-NPs. Our findings reveal that Fe2O3-NPs provide effective resistance/amelioration to arsenic toxicity by reducing arsenic availability to plants.
KW - Arsenic
KW - FeO nanoparticles
KW - Oxidative stress
KW - Vigna radiata
UR - http://www.scopus.com/inward/record.url?scp=85069917899&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2019.109496
DO - 10.1016/j.ecoenv.2019.109496
M3 - Article
C2 - 31376808
AN - SCOPUS:85069917899
SN - 0147-6513
VL - 183
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
M1 - 109496
ER -