Label-free sensitive optical detection of polychlorinated biphenyl (PCB) in an aqueous solution based on surface plasmon resonance measurements

Surin Hong; Taewook Kang; Seogil Oh; Jungwoo Moon; Inhee Choi; Kyunghee Choi; Jongheop Yi

doi:10.1016/j.snb.2008.05.006

Label-free sensitive optical detection of polychlorinated biphenyl (PCB) in an aqueous solution based on surface plasmon resonance measurements

Surin Hong, Taewook Kang, Seogil Oh, Jungwoo Moon, Inhee Choi, Kyunghee Choi, Jongheop Yi

Department of Life Science

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

A label-free, ultrasensitive method for the optical detection of polychlorinated biphenyl (PCB) is described. The detection mechanism for PCB is based on PCB-induced conformational changes of immobilized Cytochrome c (Cyt c) on an Au thin film altering the local dielectric function of the supported Cyt c, which can be detectable by surface plasmon resonance (SPR) spectroscopy. Results showed that the confirmations of Cyt c changed in a very sensitive way depend on PCB concentrations as confirmed by circular dichroism (CD) measurements and atomic force microscope (AFM) observations. Based on the results, we investigated the PCB-sensitive detection performance of the self-assembling Cyt c monolayer surface by SPR. On exposure to PCB, the reflectance R at the SPR angle of the supported Cyt c showed a sensitive and systematic increase with increasing PCB concentrations. The limit of detection was as low as 0.1 ppb and the responses completed within 10 min.

Original language	English
Pages (from-to)	300-306
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	134
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2008.05.006
State	Published - 28 Aug 2008

Keywords

Biosensor
Conformational change
Cytochrome c
Polychlorinated biphenyl
Self-assembly
Surface plasmon resonance

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10.1016/j.snb.2008.05.006

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title = "Label-free sensitive optical detection of polychlorinated biphenyl (PCB) in an aqueous solution based on surface plasmon resonance measurements",

abstract = "A label-free, ultrasensitive method for the optical detection of polychlorinated biphenyl (PCB) is described. The detection mechanism for PCB is based on PCB-induced conformational changes of immobilized Cytochrome c (Cyt c) on an Au thin film altering the local dielectric function of the supported Cyt c, which can be detectable by surface plasmon resonance (SPR) spectroscopy. Results showed that the confirmations of Cyt c changed in a very sensitive way depend on PCB concentrations as confirmed by circular dichroism (CD) measurements and atomic force microscope (AFM) observations. Based on the results, we investigated the PCB-sensitive detection performance of the self-assembling Cyt c monolayer surface by SPR. On exposure to PCB, the reflectance R at the SPR angle of the supported Cyt c showed a sensitive and systematic increase with increasing PCB concentrations. The limit of detection was as low as 0.1 ppb and the responses completed within 10 min.",

keywords = "Biosensor, Conformational change, Cytochrome c, Polychlorinated biphenyl, Self-assembly, Surface plasmon resonance",

author = "Surin Hong and Taewook Kang and Seogil Oh and Jungwoo Moon and Inhee Choi and Kyunghee Choi and Jongheop Yi",

year = "2008",

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T1 - Label-free sensitive optical detection of polychlorinated biphenyl (PCB) in an aqueous solution based on surface plasmon resonance measurements

AU - Hong, Surin

AU - Kang, Taewook

AU - Oh, Seogil

AU - Moon, Jungwoo

AU - Choi, Inhee

AU - Choi, Kyunghee

AU - Yi, Jongheop

PY - 2008/8/28

Y1 - 2008/8/28

N2 - A label-free, ultrasensitive method for the optical detection of polychlorinated biphenyl (PCB) is described. The detection mechanism for PCB is based on PCB-induced conformational changes of immobilized Cytochrome c (Cyt c) on an Au thin film altering the local dielectric function of the supported Cyt c, which can be detectable by surface plasmon resonance (SPR) spectroscopy. Results showed that the confirmations of Cyt c changed in a very sensitive way depend on PCB concentrations as confirmed by circular dichroism (CD) measurements and atomic force microscope (AFM) observations. Based on the results, we investigated the PCB-sensitive detection performance of the self-assembling Cyt c monolayer surface by SPR. On exposure to PCB, the reflectance R at the SPR angle of the supported Cyt c showed a sensitive and systematic increase with increasing PCB concentrations. The limit of detection was as low as 0.1 ppb and the responses completed within 10 min.

AB - A label-free, ultrasensitive method for the optical detection of polychlorinated biphenyl (PCB) is described. The detection mechanism for PCB is based on PCB-induced conformational changes of immobilized Cytochrome c (Cyt c) on an Au thin film altering the local dielectric function of the supported Cyt c, which can be detectable by surface plasmon resonance (SPR) spectroscopy. Results showed that the confirmations of Cyt c changed in a very sensitive way depend on PCB concentrations as confirmed by circular dichroism (CD) measurements and atomic force microscope (AFM) observations. Based on the results, we investigated the PCB-sensitive detection performance of the self-assembling Cyt c monolayer surface by SPR. On exposure to PCB, the reflectance R at the SPR angle of the supported Cyt c showed a sensitive and systematic increase with increasing PCB concentrations. The limit of detection was as low as 0.1 ppb and the responses completed within 10 min.

KW - Biosensor

KW - Conformational change

KW - Cytochrome c

KW - Polychlorinated biphenyl

KW - Self-assembly

KW - Surface plasmon resonance

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DO - 10.1016/j.snb.2008.05.006

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SN - 0925-4005

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JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

IS - 1

ER -

Label-free sensitive optical detection of polychlorinated biphenyl (PCB) in an aqueous solution based on surface plasmon resonance measurements

Abstract

Keywords

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