Mest/Peg1 inhibits Wnt signalling through regulation of LRP6 glycosylation

Hwajin Jung, Suk Kyung Lee, Eek Hoon Jho

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


Mest (mesoderm-specific transcript)/Peg1 (paternally expressed gene 1) is an imprinted gene that plays important roles in embryo development, although its biochemical role has not been determined. Ectopic expression of Mest/Peg1 inhibited Wntmediated reporter activity by enhancing the ubiquitination of β-catenin. The maturation and plasma membrane localization of the Wnt co-receptor LRP6 [LDLR (low-density lipoprotein receptor)-related protein 6], which are both necessary for Wnt signalling, were blocked by the expression of Mest/Peg1. Mest/Peg1 inhibited maturation of LRP6 by controlling the glycosylation of LRP6. Knockdown of Mest/Peg1, which might enhance Wnt signalling, blocked adipogenic differentiation of 3T3-L1 cells. Overall, our results suggest that Mest/Peg1 is a novel regulator of Wnt/β-catenin signalling during adipogenic differentiation.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalBiochemical Journal
Issue number2
StatePublished - 1 Jun 2011


  • Adipogenesis
  • Glycosylation
  • Low-density-lipoprotein-receptor-related protein 6 (LRP6)
  • Mesoderm-specific transcript/paternally expressed gene 1 (Mest/Peg1)
  • Wnt


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