Mest/Peg1 inhibits Wnt signalling through regulation of LRP6 glycosylation

Hwajin Jung; Suk Kyung Lee; Eek Hoon Jho

doi:10.1042/BJ20101512

Mest/Peg1 inhibits Wnt signalling through regulation of LRP6 glycosylation

Hwajin Jung, Suk Kyung Lee, Eek Hoon Jho

Department of Life Science

University of Seoul

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Mest (mesoderm-specific transcript)/Peg1 (paternally expressed gene 1) is an imprinted gene that plays important roles in embryo development, although its biochemical role has not been determined. Ectopic expression of Mest/Peg1 inhibited Wntmediated reporter activity by enhancing the ubiquitination of β-catenin. The maturation and plasma membrane localization of the Wnt co-receptor LRP6 [LDLR (low-density lipoprotein receptor)-related protein 6], which are both necessary for Wnt signalling, were blocked by the expression of Mest/Peg1. Mest/Peg1 inhibited maturation of LRP6 by controlling the glycosylation of LRP6. Knockdown of Mest/Peg1, which might enhance Wnt signalling, blocked adipogenic differentiation of 3T3-L1 cells. Overall, our results suggest that Mest/Peg1 is a novel regulator of Wnt/β-catenin signalling during adipogenic differentiation.

Original language	English
Pages (from-to)	263-269
Number of pages	7
Journal	Biochemical Journal
Volume	436
Issue number	2
DOIs	https://doi.org/10.1042/BJ20101512
State	Published - 1 Jun 2011

Keywords

Adipogenesis
Glycosylation
Low-density-lipoprotein-receptor-related protein 6 (LRP6)
Mesoderm-specific transcript/paternally expressed gene 1 (Mest/Peg1)
Wnt

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title = "Mest/Peg1 inhibits Wnt signalling through regulation of LRP6 glycosylation",

abstract = "Mest (mesoderm-specific transcript)/Peg1 (paternally expressed gene 1) is an imprinted gene that plays important roles in embryo development, although its biochemical role has not been determined. Ectopic expression of Mest/Peg1 inhibited Wntmediated reporter activity by enhancing the ubiquitination of β-catenin. The maturation and plasma membrane localization of the Wnt co-receptor LRP6 [LDLR (low-density lipoprotein receptor)-related protein 6], which are both necessary for Wnt signalling, were blocked by the expression of Mest/Peg1. Mest/Peg1 inhibited maturation of LRP6 by controlling the glycosylation of LRP6. Knockdown of Mest/Peg1, which might enhance Wnt signalling, blocked adipogenic differentiation of 3T3-L1 cells. Overall, our results suggest that Mest/Peg1 is a novel regulator of Wnt/β-catenin signalling during adipogenic differentiation.",

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author = "Hwajin Jung and Lee, {Suk Kyung} and Jho, {Eek Hoon}",

year = "2011",

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AU - Jung, Hwajin

AU - Lee, Suk Kyung

AU - Jho, Eek Hoon

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N2 - Mest (mesoderm-specific transcript)/Peg1 (paternally expressed gene 1) is an imprinted gene that plays important roles in embryo development, although its biochemical role has not been determined. Ectopic expression of Mest/Peg1 inhibited Wntmediated reporter activity by enhancing the ubiquitination of β-catenin. The maturation and plasma membrane localization of the Wnt co-receptor LRP6 [LDLR (low-density lipoprotein receptor)-related protein 6], which are both necessary for Wnt signalling, were blocked by the expression of Mest/Peg1. Mest/Peg1 inhibited maturation of LRP6 by controlling the glycosylation of LRP6. Knockdown of Mest/Peg1, which might enhance Wnt signalling, blocked adipogenic differentiation of 3T3-L1 cells. Overall, our results suggest that Mest/Peg1 is a novel regulator of Wnt/β-catenin signalling during adipogenic differentiation.

AB - Mest (mesoderm-specific transcript)/Peg1 (paternally expressed gene 1) is an imprinted gene that plays important roles in embryo development, although its biochemical role has not been determined. Ectopic expression of Mest/Peg1 inhibited Wntmediated reporter activity by enhancing the ubiquitination of β-catenin. The maturation and plasma membrane localization of the Wnt co-receptor LRP6 [LDLR (low-density lipoprotein receptor)-related protein 6], which are both necessary for Wnt signalling, were blocked by the expression of Mest/Peg1. Mest/Peg1 inhibited maturation of LRP6 by controlling the glycosylation of LRP6. Knockdown of Mest/Peg1, which might enhance Wnt signalling, blocked adipogenic differentiation of 3T3-L1 cells. Overall, our results suggest that Mest/Peg1 is a novel regulator of Wnt/β-catenin signalling during adipogenic differentiation.

KW - Adipogenesis

KW - Glycosylation

KW - Low-density-lipoprotein-receptor-related protein 6 (LRP6)

KW - Mesoderm-specific transcript/paternally expressed gene 1 (Mest/Peg1)

KW - Wnt

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JO - Biochemical Journal

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ER -

Mest/Peg1 inhibits Wnt signalling through regulation of LRP6 glycosylation

Abstract

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