Metabolic engineering of deinococcus radiodurans for the production of phytoene

Sun Wook Jeong, Chang Keun Kang, Yong Jun Choi

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A metabolically-engineered Deinococcus radiodurans R1 strain capable of producing phytoene, a colorless C 40 carotenoid and a promising antioxidant, has been developed. To make this base strain, first, the crtI gene encoding phytoene desaturase was deleted to block the conversion of phytoene to other carotenoids such as lycopene and γ-carotene. This engineered strain produced 0.413 ± 0.023 mg/l of phytoene from 10 g/l of fructose. Further enhanced production of phytoene up to 4.46 ± 0.19 mg/l was achieved by overexpressing the crtB gene encoding phytoene synthase and the dxs genes encoding 1-deoxy-D-xylulose-5-phosphate synthase gene, and by deleting the crtD gene. High cell-density culture of our final engineered strain allowed production of 10.3 ± 0.85 mg/l of phytoene with the yield and productivity of 1.04 ± 0.05 mg/g and 0.143 ± 0.012 mg/l/h, respectively, from 10 g/l of fructose. Furthermore, the antioxidant potential of phytoene produced by the final engineered strain was confirmed by in vitro DPPH radical-scavenging assay.

Original languageEnglish
Pages (from-to)1691-1699
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume28
Issue number10
DOIs
StatePublished - Oct 2018

Keywords

  • Antioxidation
  • Deinococcus radiodurans
  • Metabolic engineering
  • Phytoene

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