Metabolic engineering of Escherichia coli for the production of 1-propanol

Yong Jun Choi, Jin Hwan Park, Tae Yong Kim, Sang Yup Lee

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

An engineered Escherichia coli strain that produces 1-propanol under aerobic condition was developed based on an l-threonine-overproducing E. coli strain. First, a feedback resistant ilvA gene encoding threonine dehydratase was introduced and the competing metabolic pathway genes were deleted. Further engineering was performed by overexpressing the cimA gene encoding citramalate synthase and the ackA gene encoding acetate kinase A/propionate kinase II, introducing a modified adhE gene encoding an aerobically functional AdhE, and by deleting the rpoS gene encoding the stationary phase sigma factor. Fed-batch culture of the final engineered strain harboring pBRthrABC-tac-cimA-tac-ackA and pTacDA-tac-adhEmut allowed production of 10.8gL-1 of 1-propanol with the yield and productivity of 0.107gg-1 and 0.144gL-1h-1, respectively, from 100gL-1 of glucose, and 10.3gL-1 of 1-propanol with the yield and productivity of 0.259gg-1 and 0.083gL-1h-1, respectively, from 40gL-1 glycerol.

Original languageEnglish
Pages (from-to)477-486
Number of pages10
JournalMetabolic Engineering
Volume14
Issue number5
DOIs
StatePublished - Sep 2012

Keywords

  • 1-propanol
  • 2-ketobutyrate
  • Escherichia coli
  • Glycerol
  • In silico metabolic flux analysis
  • L-threonine

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