Abstract
A novel rice (Oryza sativa L.) gene, homologous to Arabidopsis pathogenesis-related NDR1 gene, was cloned from cDNA library prepared from 30 min Magnaporthe grisea -treated rice seedling leaves, and named as OsNDRl. OsNDRl encoded a 220-amino-acid polypeptide and was highly similar to the Arabidopsis AtNDRl protein. OsNDRl is a plasma membrane (PM)-localized protein, and presumes through sequence analysis and protein localization experiment. Overexpression of OsNDRl promotes the expression of PBZ1 that is essential for the activation of defense/stress-related gene. The OsNDRl promoter did not respond significantly to treatments with either SA, PBZ, or ETP. Exogenously applied BTH induces the same set of SAR genes as biological induction, providing further evidence for BTH as a signal. Presumably, BTH is bound by a receptor and the binding triggers a signal transduction cascade that has an ultimate effect on transcription factors that regulate SAR gene expression. Thus OsNDRl may act as a transducer of pathogen signals and/or interact with the pathogen and is indeed another important step in clarifying the component participating in the defense response pathways in rice.
Original language | English |
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Pages (from-to) | 149-157 |
Number of pages | 9 |
Journal | Plant Pathology Journal |
Volume | 21 |
Issue number | 2 |
DOIs | |
State | Published - 2005 |
Keywords
- Benzo(l,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH)
- Magnaporthe grisea
- Oryza sativa L.
- OsNDRl
- System acquired resistance (SAR)