Molecular cloning and functional analysis of rice (Oryza sativa L.) OsNDRl on defense signaling pathway

Joo Hee Lee, Sun Hyung Kim, Young Ho Jung, Jung A. Kim, Mi Ok Lee, Pil Gyu Choi, Woobong Choi, Kyung Nam Kim, Nam Soo Jwa

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

A novel rice (Oryza sativa L.) gene, homologous to Arabidopsis pathogenesis-related NDR1 gene, was cloned from cDNA library prepared from 30 min Magnaporthe grisea -treated rice seedling leaves, and named as OsNDRl. OsNDRl encoded a 220-amino-acid polypeptide and was highly similar to the Arabidopsis AtNDRl protein. OsNDRl is a plasma membrane (PM)-localized protein, and presumes through sequence analysis and protein localization experiment. Overexpression of OsNDRl promotes the expression of PBZ1 that is essential for the activation of defense/stress-related gene. The OsNDRl promoter did not respond significantly to treatments with either SA, PBZ, or ETP. Exogenously applied BTH induces the same set of SAR genes as biological induction, providing further evidence for BTH as a signal. Presumably, BTH is bound by a receptor and the binding triggers a signal transduction cascade that has an ultimate effect on transcription factors that regulate SAR gene expression. Thus OsNDRl may act as a transducer of pathogen signals and/or interact with the pathogen and is indeed another important step in clarifying the component participating in the defense response pathways in rice.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalPlant Pathology Journal
Volume21
Issue number2
DOIs
StatePublished - 2005

Keywords

  • Benzo(l,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH)
  • Magnaporthe grisea
  • Oryza sativa L.
  • OsNDRl
  • System acquired resistance (SAR)

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