Pollen allergic risk assessment of genetically modified virus resistant pepper and functional Chinese cabbage

Ju Suk Lee, Ye Jin Kim, Ki Hyun Ryu, Tae Ho Han, Kuen Woo Park, Kyu Hwan Chung, Chan Lee, Gung Pyo Lee, Sun Hyung Kim, Jin Sung Hong, Young Doo Park, Eun Taeck Woo, Sung Chul Park, Dae Yeul Son

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Pollen of genetically modified (GM) pepper containing the gene for cucumber mosaic virus (CMV) coat protein (CP) and GM Chinese cabbage with high phenylethylisothiocyanate (PEITC) content was investigated for assessment of allergic risk. Amino acid (AA) sequences of the inserted gene products of GM virus resistant pepper and GM Chinese cabbage with high PEITC content were compared with those of known allergens. No known allergen greater than 35% AA sequence homology, over 80 AA window or more than 8 consecutive identical AA was found. Protein patterns of GM/non-GM pepper and Chinese cabbage pollen extracts in SDS-PAGE analysis showed the same distribution of protein bands among the GM and non-GM pepper or Chinese cabbage, respectively. Sera from pollen allergic patients showed some IgE reactivity via immunoblotting and ELISA; however, no differences were observed between the pollen of GM and non-GM pepper or Chinese cabbage, respectively. Based on these results, we conclude that pollens of the virus resistant GM pepper and GM Chinese cabbage with high PEITC have no differences in their protein composition or allergenicity relative to non-GM pepper and Chinese cabbage.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalHorticulture Environment and Biotechnology
Volume53
Issue number2
DOIs
StatePublished - Apr 2012

Keywords

  • GM
  • allergy
  • cucumber mosaic virus (CMV)
  • phenylethylisothiocyanate (PEITC)

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