Preparation of a self-assembled cytochrome c monolayer on a gold substrate for biomolecular device architecture

The segregation ability of 3-[(3-cholamidopropyl) dimethylammonio]-1- propanesulfonate (CHAPS), a zwitterionic surfactant, on cytochrome c (cyt c) aggregates in a phosphate buffer solution was quantified through the dynamic light-scattering analysis, and CHAPS was found to have an excellent ability in reducing nonspecific affinity among cyt c molecules. When CHAPS was applied to cyt c aggregates on the surface of gold substrates modified with self-assembled cyt c monolayer, the aggregates were found to be successfully eliminated by high-resolution atomic force microscopy image with 30-nm-sized cyt c clusters. This technique is expected to be useful to prepare a self-assembled monolayer of metalloproteins without their aggregates which may degrade the electrochemical property required as a biomolecular electronic device.