Promoter characterization of the rat Na+/I- symporter gene

Qiang Tong; Kwon Yul Ryu; Sissy M. Jhiang

doi:10.1006/bbrc.1997.7432

Promoter characterization of the rat Na⁺/I^- symporter gene

Qiang Tong, Kwon Yul Ryu, Sissy M. Jhiang

Department of Life Science

Ohio State University

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

The Na⁺/I^- symporter is the molecule that mediates active iodide uptake in the thyroid gland. A 16.4 kb genomic DNA fragment of the rat Na⁺/I^- symporter gene (rNIS) was isolated, restriction mapped and a 2 kb region immediate 5' to the ATG site was sequenced. The transcription start site for rNIS was mapped to -98 nucleotide (nt) relative to the ATG translation initiation site. A series of 5' genomic DNA fragments ranging from 233 bp to 8 kb were tested for promoter activity in both thyroid and non-thyroid cells. Our results indicate that the DNA regulatory elements within 8 kb of the 5' flanking region of rNIS are not sufficient to confer thyroid-selective transcription. However, consistent with the clinical observation that NIS expression is reduced in thyroid tumors, the rNIS promoter activity is suppressed in ret/PTC1 transformed NIH3T3 cells, compared to non-transformed NIH3T3 cells.

Original language	English
Pages (from-to)	34-41
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	239
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1997.7432
State	Published - 9 Oct 1997

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title = "Promoter characterization of the rat Na+/I- symporter gene",

abstract = "The Na+/I- symporter is the molecule that mediates active iodide uptake in the thyroid gland. A 16.4 kb genomic DNA fragment of the rat Na+/I- symporter gene (rNIS) was isolated, restriction mapped and a 2 kb region immediate 5' to the ATG site was sequenced. The transcription start site for rNIS was mapped to -98 nucleotide (nt) relative to the ATG translation initiation site. A series of 5' genomic DNA fragments ranging from 233 bp to 8 kb were tested for promoter activity in both thyroid and non-thyroid cells. Our results indicate that the DNA regulatory elements within 8 kb of the 5' flanking region of rNIS are not sufficient to confer thyroid-selective transcription. However, consistent with the clinical observation that NIS expression is reduced in thyroid tumors, the rNIS promoter activity is suppressed in ret/PTC1 transformed NIH3T3 cells, compared to non-transformed NIH3T3 cells.",

author = "Qiang Tong and Ryu, \{Kwon Yul\} and Jhiang, \{Sissy M.\}",

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AU - Ryu, Kwon Yul

AU - Jhiang, Sissy M.

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AB - The Na+/I- symporter is the molecule that mediates active iodide uptake in the thyroid gland. A 16.4 kb genomic DNA fragment of the rat Na+/I- symporter gene (rNIS) was isolated, restriction mapped and a 2 kb region immediate 5' to the ATG site was sequenced. The transcription start site for rNIS was mapped to -98 nucleotide (nt) relative to the ATG translation initiation site. A series of 5' genomic DNA fragments ranging from 233 bp to 8 kb were tested for promoter activity in both thyroid and non-thyroid cells. Our results indicate that the DNA regulatory elements within 8 kb of the 5' flanking region of rNIS are not sufficient to confer thyroid-selective transcription. However, consistent with the clinical observation that NIS expression is reduced in thyroid tumors, the rNIS promoter activity is suppressed in ret/PTC1 transformed NIH3T3 cells, compared to non-transformed NIH3T3 cells.

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Promoter characterization of the rat Na⁺/I^- symporter gene

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