Rapid and reliable method of extracting DNA and RNA from sweetpotato, Ipomoea batatas (L). Lam

Sun Hyung Kim, Tatsuro Hamada

Research output: Contribution to journalArticlepeer-review

124 Scopus citations

Abstract

A quick, simple and reliable method of extracting DNA from sweetpotato (Ipomoea batatas (L.) Lam.) has been developed. The method was applied successfully for extraction of total DNA from leaves and total RNA from leaves and various tissues. The yield of DNA extracted by this procedure was high (about 1 mg/g leaf tissue). The extracted DNA was completely digested by restriction endonucleases indicating the absence of common contaminating compounds. The absorbancy ratios of A260/A230 and A260/A280 of isolated RNA were approx. 2 and the yield was about 0.2 mg/g fresh wt. CIPK and tublin genes were successfully amplified by RT-PCR, suggesting the integrity of isolated RNA. The total DNA and RNA isolated by this method was of sufficient quality for subsequent molecular analysis.

Original languageEnglish
Pages (from-to)1841-1845
Number of pages5
JournalBiotechnology Letters
Volume27
Issue number23-24
DOIs
StatePublished - Dec 2005

Keywords

  • DNA
  • RNA
  • Sweetpotato (Ipomoea batatas (L.) Lam.)

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