Rational design of paraoxonase 1 (PON1) for the efficient hydrolysis of organophosphates

Quang Anh Tuan Le; Rakwoo Chang; Yong Hwan Kim

doi:10.1039/c5cc05857k

Rational design of paraoxonase 1 (PON1) for the efficient hydrolysis of organophosphates

Quang Anh Tuan Le, Rakwoo Chang, Yong Hwan Kim

Department of Applied Chemistry

Kwangwoon University

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A rational design of paraoxonase 1 based on molecular docking discovered H115W/T332S and I74F/H115W/T332S mutants exhibited a 40-fold increase in catalytic efficiency (k_cat/K_m) toward the hydrolysis of two toxic and popular organophosphates (diethyl-paraoxon and dimethyl-paraoxon). Moreover, the conversion of the paraoxons (741.3-825.6 mg L^-1) by the evolved mutants was 42-60-fold faster than that by the wild type.

Original language	English
Pages (from-to)	14536-14539
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	77
DOIs	https://doi.org/10.1039/c5cc05857k
State	Published - 12 Aug 2015

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title = "Rational design of paraoxonase 1 (PON1) for the efficient hydrolysis of organophosphates",

abstract = "A rational design of paraoxonase 1 based on molecular docking discovered H115W/T332S and I74F/H115W/T332S mutants exhibited a 40-fold increase in catalytic efficiency (kcat/Km) toward the hydrolysis of two toxic and popular organophosphates (diethyl-paraoxon and dimethyl-paraoxon). Moreover, the conversion of the paraoxons (741.3-825.6 mg L-1) by the evolved mutants was 42-60-fold faster than that by the wild type.",

author = "Le, {Quang Anh Tuan} and Rakwoo Chang and Kim, {Yong Hwan}",

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AU - Le, Quang Anh Tuan

AU - Chang, Rakwoo

AU - Kim, Yong Hwan

N1 - Publisher Copyright: © The Royal Society of Chemistry.

PY - 2015/8/12

Y1 - 2015/8/12

N2 - A rational design of paraoxonase 1 based on molecular docking discovered H115W/T332S and I74F/H115W/T332S mutants exhibited a 40-fold increase in catalytic efficiency (kcat/Km) toward the hydrolysis of two toxic and popular organophosphates (diethyl-paraoxon and dimethyl-paraoxon). Moreover, the conversion of the paraoxons (741.3-825.6 mg L-1) by the evolved mutants was 42-60-fold faster than that by the wild type.

AB - A rational design of paraoxonase 1 based on molecular docking discovered H115W/T332S and I74F/H115W/T332S mutants exhibited a 40-fold increase in catalytic efficiency (kcat/Km) toward the hydrolysis of two toxic and popular organophosphates (diethyl-paraoxon and dimethyl-paraoxon). Moreover, the conversion of the paraoxons (741.3-825.6 mg L-1) by the evolved mutants was 42-60-fold faster than that by the wild type.

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U2 - 10.1039/c5cc05857k

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SP - 14536

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JO - Chemical Communications

JF - Chemical Communications

IS - 77

ER -

Rational design of paraoxonase 1 (PON1) for the efficient hydrolysis of organophosphates

Abstract

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