Rational design of paraoxonase 1 (PON1) for the efficient hydrolysis of organophosphates

Quang Anh Tuan Le, Rakwoo Chang, Yong Hwan Kim

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A rational design of paraoxonase 1 based on molecular docking discovered H115W/T332S and I74F/H115W/T332S mutants exhibited a 40-fold increase in catalytic efficiency (kcat/Km) toward the hydrolysis of two toxic and popular organophosphates (diethyl-paraoxon and dimethyl-paraoxon). Moreover, the conversion of the paraoxons (741.3-825.6 mg L-1) by the evolved mutants was 42-60-fold faster than that by the wild type.

Original languageEnglish
Pages (from-to)14536-14539
Number of pages4
JournalChemical Communications
Volume51
Issue number77
DOIs
StatePublished - 12 Aug 2015

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