Abstract
Elution ability of 3-[(3-cholamidopropyl) dimethylammonio]-l-propanesulfonate (CHAPS) was found to segregate readily the aggregates of ferredoxin from its self-assembled monolayer onto metal substrates without a loss of the intrinsic property of ferredoxin: a high-resolution AFM image showed that the self-assembled ferredoxin monolayer on the (100) surface of the silicon substrate consisted of ferredoxin monomers or small clusters when the surface was treated with CHAPS. The electric property of the monolayer of ferredoxin on the gold surface was also confirmed to remain intact by the cyclic voltammetry measurements. Those results offer a new technique for the elimination of nonspecific adsorption of proteins on inorganic surfaces, which has been a long-term problem in the fabrication of biomolecular electronic devices.
Original language | English |
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Pages (from-to) | 243-247 |
Number of pages | 5 |
Journal | Journal of Industrial and Engineering Chemistry |
Volume | 9 |
Issue number | 3 |
State | Published - 2003 |
Keywords
- Biomolecular electronic device
- CHAPS
- Ferredoxin
- Self-assembled monolayer