Self-assembled DNA hollow spheres from microsponges

We reportanovel approach for generating nanosized DNA hollow spheres (HSs) using enzymatically produced DNA microsponges in aself-templating manner. Inprevious studies, preparation of DNA nanostructures with specified functions required multiple complicated steps. In this study, however, a simple treatment with the nucleophilic agent 4-dimethylaminopyridine (DMAP) enabledagradual disentanglement of DNA in microsponges byelectrostatic interactions between DMAP and DNA, and the DNA underwent areassembly processto generate hollow shell structures without denaturation/annealing by thermal cycling. In addition, this synthetic process was conducted in a water-based system without organic solvents, enabling the synthesis of biologically and environmentally friendly products. Based on the benefits of hollow shell structures, which include their high surface-to-volume ratio and ability to encapsulate small molecules, we envision that this simple approach for synthesizing DNA HSs will provideanew platform for maximizing their potential useindrug delivery and bio-imaging.