Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression

Hyejin Kim, Yongkuk Park, Jong Bum Lee

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Although mRNA has several advantages over plasmid DNA when delivered into cells for gene expression, mRNA transfection is a very rare occurrence in gene delivery. This is mainly because of the labile nature of RNA, resulting in a low expression level of the desired protein. In this study, self-assembled mRNA nanoparticles (mRNA-NPs) packed with multiple repeats of mRNA were synthesized to achieve efficient gene expression. This approach required only a one-step process to synthesize particles with a minimal amount of plasmid DNA to produce the RNA transcripts via rolling circle transcription. Moreover, there are no concerns for cytotoxicity which can be caused by chemical condensates because mRNA-NPs are made entirely of mRNA. An examination of the cells transfected with the mRNA-NPs encoding the green fluorescence protein (GFP) confirmed that the mRNA-NPs can be used as a novel platform for effective gene delivery.

Original languageEnglish
Article number12737
JournalScientific Reports
StatePublished - 3 Aug 2015


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