Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production

Hyangsu Nam; Taehyeon Kim; Sunghyun Moon; Yoonbin Ji; Jong Bum Lee

doi:10.1016/j.isci.2023.107089

Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production

Hyangsu Nam, Taehyeon Kim, Sunghyun Moon, Yoonbin Ji, Jong Bum Lee

Department of Chemical Engineering

University of Seoul

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a multimeric genomic DNA hydrogel (mGD-gel) via rolling circle chain amplification (RCCA) using dual single-stranded circular plasmids with multiple primers. The mGD-gel exhibited significantly enhanced protein yield. In addition, mGD-gel can be reused at least five times, and the shape of the mGD-gel can be easily manipulated without losing the feasibility of protein expression. The mGD-gel platform based on the self-assembly of multimeric genomic DNA strands (mGD strands) has the potential to be used in CFPS systems for a variety of biotechnological applications.

Original language	English
Article number	107089
Journal	iScience
Volume	26
Issue number	7
DOIs	https://doi.org/10.1016/j.isci.2023.107089
State	Published - 21 Jul 2023

Keywords

Biotechnology
Molecular self-assembly
Scaffolds in supramolecular chemistry

Access to Document

10.1016/j.isci.2023.107089

Cite this

@article{b4598d5a4a35466396c730b70b62199a,

title = "Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production",

abstract = "Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a multimeric genomic DNA hydrogel (mGD-gel) via rolling circle chain amplification (RCCA) using dual single-stranded circular plasmids with multiple primers. The mGD-gel exhibited significantly enhanced protein yield. In addition, mGD-gel can be reused at least five times, and the shape of the mGD-gel can be easily manipulated without losing the feasibility of protein expression. The mGD-gel platform based on the self-assembly of multimeric genomic DNA strands (mGD strands) has the potential to be used in CFPS systems for a variety of biotechnological applications.",

keywords = "Biotechnology, Molecular self-assembly, Scaffolds in supramolecular chemistry",

author = "Hyangsu Nam and Taehyeon Kim and Sunghyun Moon and Yoonbin Ji and Lee, \{Jong Bum\}",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

month = jul,

day = "21",

doi = "10.1016/j.isci.2023.107089",

language = "English",

volume = "26",

journal = "iScience",

issn = "2589-0042",

publisher = "Elsevier Inc.",

number = "7",

}

TY - JOUR

T1 - Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production

AU - Nam, Hyangsu

AU - Kim, Taehyeon

AU - Moon, Sunghyun

AU - Ji, Yoonbin

AU - Lee, Jong Bum

PY - 2023/7/21

Y1 - 2023/7/21

N2 - Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a multimeric genomic DNA hydrogel (mGD-gel) via rolling circle chain amplification (RCCA) using dual single-stranded circular plasmids with multiple primers. The mGD-gel exhibited significantly enhanced protein yield. In addition, mGD-gel can be reused at least five times, and the shape of the mGD-gel can be easily manipulated without losing the feasibility of protein expression. The mGD-gel platform based on the self-assembly of multimeric genomic DNA strands (mGD strands) has the potential to be used in CFPS systems for a variety of biotechnological applications.

AB - Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a multimeric genomic DNA hydrogel (mGD-gel) via rolling circle chain amplification (RCCA) using dual single-stranded circular plasmids with multiple primers. The mGD-gel exhibited significantly enhanced protein yield. In addition, mGD-gel can be reused at least five times, and the shape of the mGD-gel can be easily manipulated without losing the feasibility of protein expression. The mGD-gel platform based on the self-assembly of multimeric genomic DNA strands (mGD strands) has the potential to be used in CFPS systems for a variety of biotechnological applications.

KW - Biotechnology

KW - Molecular self-assembly

KW - Scaffolds in supramolecular chemistry

UR - https://www.scopus.com/pages/publications/85163219525

U2 - 10.1016/j.isci.2023.107089

DO - 10.1016/j.isci.2023.107089

M3 - Article

AN - SCOPUS:85163219525

SN - 2589-0042

VL - 26

JO - iScience

JF - iScience

IS - 7

M1 - 107089

ER -

Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this