Starch-branching enzyme I gene (IbSBEI) from sweet potato (Ipomoea batatas); molecular cloning and expression analysis

Tatsuro Hamada; Sun Hyung Kim; Takiko Shimada

doi:10.1007/s10529-006-9083-x

Starch-branching enzyme I gene (IbSBEI) from sweet potato (Ipomoea batatas); molecular cloning and expression analysis

Tatsuro Hamada, Sun Hyung Kim, Takiko Shimada

Department of Environmental Horticulture

Ishikawa Prefectural University

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The cDNA of the starch-branching enzyme I gene (IbSBEI) in the sweet potato (Ipomoea batatas) has been cloned and sequenced. The IbSBEI amino acid sequence was 81% identical to that of potato StSBEI. DNA gel-blot analyses demonstrated that at least two copies of IbSBEI are present in the sweet potato genome. IbSBEI was strongly expressed in tuberous roots. Transcript levels in the roots of single leaf cuttings were extremely low during the first 15-40 d after planting and continuously increased up to 50 d, by which time the tuberous roots had almost completely developed. This indicates that IbSBEI may work in concert with the AGPase large subunit during the primary phase of starch granule formation.

Original language	English
Pages (from-to)	1255-1261
Number of pages	7
Journal	Biotechnology Letters
Volume	28
Issue number	16
DOIs	https://doi.org/10.1007/s10529-006-9083-x
State	Published - Aug 2006

Keywords

Ipomoea batatas
Starch
Starch branching enzyme
Sweet potato

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10.1007/s10529-006-9083-x

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title = "Starch-branching enzyme I gene (IbSBEI) from sweet potato (Ipomoea batatas); molecular cloning and expression analysis",

abstract = "The cDNA of the starch-branching enzyme I gene (IbSBEI) in the sweet potato (Ipomoea batatas) has been cloned and sequenced. The IbSBEI amino acid sequence was 81% identical to that of potato StSBEI. DNA gel-blot analyses demonstrated that at least two copies of IbSBEI are present in the sweet potato genome. IbSBEI was strongly expressed in tuberous roots. Transcript levels in the roots of single leaf cuttings were extremely low during the first 15-40 d after planting and continuously increased up to 50 d, by which time the tuberous roots had almost completely developed. This indicates that IbSBEI may work in concert with the AGPase large subunit during the primary phase of starch granule formation.",

keywords = "Ipomoea batatas, Starch, Starch branching enzyme, Sweet potato",

author = "Tatsuro Hamada and Kim, {Sun Hyung} and Takiko Shimada",

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T1 - Starch-branching enzyme I gene (IbSBEI) from sweet potato (Ipomoea batatas); molecular cloning and expression analysis

AU - Hamada, Tatsuro

AU - Kim, Sun Hyung

AU - Shimada, Takiko

PY - 2006/8

Y1 - 2006/8

N2 - The cDNA of the starch-branching enzyme I gene (IbSBEI) in the sweet potato (Ipomoea batatas) has been cloned and sequenced. The IbSBEI amino acid sequence was 81% identical to that of potato StSBEI. DNA gel-blot analyses demonstrated that at least two copies of IbSBEI are present in the sweet potato genome. IbSBEI was strongly expressed in tuberous roots. Transcript levels in the roots of single leaf cuttings were extremely low during the first 15-40 d after planting and continuously increased up to 50 d, by which time the tuberous roots had almost completely developed. This indicates that IbSBEI may work in concert with the AGPase large subunit during the primary phase of starch granule formation.

AB - The cDNA of the starch-branching enzyme I gene (IbSBEI) in the sweet potato (Ipomoea batatas) has been cloned and sequenced. The IbSBEI amino acid sequence was 81% identical to that of potato StSBEI. DNA gel-blot analyses demonstrated that at least two copies of IbSBEI are present in the sweet potato genome. IbSBEI was strongly expressed in tuberous roots. Transcript levels in the roots of single leaf cuttings were extremely low during the first 15-40 d after planting and continuously increased up to 50 d, by which time the tuberous roots had almost completely developed. This indicates that IbSBEI may work in concert with the AGPase large subunit during the primary phase of starch granule formation.

KW - Ipomoea batatas

KW - Starch

KW - Starch branching enzyme

KW - Sweet potato

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DO - 10.1007/s10529-006-9083-x

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JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Starch-branching enzyme I gene (IbSBEI) from sweet potato (Ipomoea batatas); molecular cloning and expression analysis

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Keywords

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