Syntrophic metabolism facilitates Methanosarcina-led methanation in the anaerobic digestion of lipidic slaughterhouse waste

Shouvik Saha; Mayur B. Kurade; Geon Soo Ha; Sean S. Lee; Hyun Seog Roh; Young Kwon Park; Byong Hun Jeon

doi:10.1016/j.biortech.2021.125250

Syntrophic metabolism facilitates Methanosarcina-led methanation in the anaerobic digestion of lipidic slaughterhouse waste

Shouvik Saha, Mayur B. Kurade, Geon Soo Ha, Sean S. Lee, Hyun Seog Roh, Young Kwon Park, Byong Hun Jeon

School of Environmental Engineering

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Different inoculum to slaughterhouse waste (SHW) ratios (Ino/SHW) influences the digester performance, substrate utilization, and methane yield through microbial shift and their metabolic syntrophy. Acetoclastic Methanosarcina (68–87%) was dominant in the exponential phase, overpowering the initial abundance of Methanosaeta (86% of methanogens) in the SHW digesters. Positive interactions among acetogenic and acetate-oxidizing species of Clostridium (11%) with Methanosarcina (84% of methanogens) improved the methanogenic activity (292 mL g⁻¹ VS_initial d⁻¹) and final VS utilization (90%) at the highest Ino/SHW loading. In contrast, significant improvement of methane yield (152% higher than the control) at the lowest Ino/SHW loading was attributed to strong syntrophy among Methanosaeta (24% of methanogens) and its exoelectrogenic partners, Bythopirellula (0.52%) and Mariniphaga (0.08%) and the acetogenic Cloacimonas (0.16%) and Longilinea (0.32%). These syntrophic interactions among the core microbiota induced major metabolic activities, including butanoate, glycine, serine and threonine, methane, propanoate, and pyruvate metabolism, and quorum sensing.

Original language	English
Article number	125250
Journal	Bioresource Technology
Volume	335
DOIs	https://doi.org/10.1016/j.biortech.2021.125250
State	Published - Sep 2021

Keywords

Acetoclastic methanogenesis
Anaerobic digestion
Methane
Microbial symbiosis
Slaughterhouse waste

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2021.125250

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title = "Syntrophic metabolism facilitates Methanosarcina-led methanation in the anaerobic digestion of lipidic slaughterhouse waste",

abstract = "Different inoculum to slaughterhouse waste (SHW) ratios (Ino/SHW) influences the digester performance, substrate utilization, and methane yield through microbial shift and their metabolic syntrophy. Acetoclastic Methanosarcina (68–87%) was dominant in the exponential phase, overpowering the initial abundance of Methanosaeta (86% of methanogens) in the SHW digesters. Positive interactions among acetogenic and acetate-oxidizing species of Clostridium (11%) with Methanosarcina (84% of methanogens) improved the methanogenic activity (292 mL g−1 VSinitial d−1) and final VS utilization (90%) at the highest Ino/SHW loading. In contrast, significant improvement of methane yield (152% higher than the control) at the lowest Ino/SHW loading was attributed to strong syntrophy among Methanosaeta (24% of methanogens) and its exoelectrogenic partners, Bythopirellula (0.52%) and Mariniphaga (0.08%) and the acetogenic Cloacimonas (0.16%) and Longilinea (0.32%). These syntrophic interactions among the core microbiota induced major metabolic activities, including butanoate, glycine, serine and threonine, methane, propanoate, and pyruvate metabolism, and quorum sensing.",

keywords = "Acetoclastic methanogenesis, Anaerobic digestion, Methane, Microbial symbiosis, Slaughterhouse waste",

author = "Shouvik Saha and Kurade, {Mayur B.} and Ha, {Geon Soo} and Lee, {Sean S.} and Roh, {Hyun Seog} and Park, {Young Kwon} and Jeon, {Byong Hun}",

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AU - Saha, Shouvik

AU - Kurade, Mayur B.

AU - Ha, Geon Soo

AU - Lee, Sean S.

AU - Roh, Hyun Seog

AU - Park, Young Kwon

AU - Jeon, Byong Hun

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KW - Acetoclastic methanogenesis

KW - Anaerobic digestion

KW - Methane

KW - Microbial symbiosis

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Syntrophic metabolism facilitates Methanosarcina-led methanation in the anaerobic digestion of lipidic slaughterhouse waste

Abstract

Keywords

UN SDGs

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