The X-ray crystal structure of PA1374 from Pseudomonas aeruginosa, a putative oxidative-stress sensing transcriptional regulator

Hyunjin Kim, Jungwoo Choe

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Members of the multiple antibiotic resistance regulator (MarR) family regulate the expression of genes related to antibiotic resistance, oxidative stress, and virulence in bacteria and Archaea. Here, we determined the structure of PA1374 from Pseudomonas aeruginosa at 2.3. å resolution. PA1374 belonged to the MarR family and its structure revealed a tightly bound dimer with each subunit containing a winged helix-turn-helix (wHTH) DNA-binding motif. Conserved arginine residues, Arg55, Arg74, and Arg77, were located in the wHTH region, which might be important to DNA binding. Furthermore, each monomer contained a pocket made of conserved hydrophobic residues. A highly conserved Cys11 located at one end of this pocket may undergo oxidation by organic hydroperoxide molecules, as shown in other MarR family proteins acting as redox-sensing regulators. These results provide insights about the role of PA1374 as a putative oxidative-stress sensing transcriptional regulator.

Original languageEnglish
Pages (from-to)376-381
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume431
Issue number3
DOIs
StatePublished - 15 Feb 2013

Keywords

  • Crystallography
  • MarR family
  • Oxidative stress
  • Transcriptional regulator

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