Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]: Molecular cloning and functional analysis

Sang Gyu Seo; Ji Seong Kim; Seung Won Kang; Mi Rae Shin; You Sun Yang; Gung Pyo Lee; Jin Sung Hong; Sun Hyung Kim

doi:10.1007/s11816-008-0072-5

Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]: Molecular cloning and functional analysis

Sang Gyu Seo, Ji Seong Kim, Seung Won Kang, Mi Rae Shin, You Sun Yang, Gung Pyo Lee, Jin Sung Hong, Sun Hyung Kim

Department of Environmental Horticulture

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The cDNA of the touch-induced genes (TCH) of the sweet potato [Ipomoea batatas (L.) Lam.] has been cloned and analyzed. IbTCH1, which exists as at least two-copy genes in the genome of the sweet potato, encodes for 148-amino acid polypeptides, and harbors four conversed Ca²⁺-binding motif EF-hands. IbTCH1 was shown to be expressed in the flower, leaf, thick pigmented root, and particularly in the white fibrous root, but expressed only weakly in the petiole. IbTCH1 is upregulated upon exposure to environmental stresses, dehydration, and jasmonic acid. Furthermore, IbTCH1 is developmentally regulated in the leaf and root. These results strongly indicate that the gene performs functions in both plant development and in defense/stress-signaling pathways.

Original language	English
Pages (from-to)	253-258
Number of pages	6
Journal	Plant Biotechnology Reports
Volume	2
Issue number	4
DOIs	https://doi.org/10.1007/s11816-008-0072-5
State	Published - Nov 2008

Keywords

CaM
Dehydration
Sweet potato [Ipomoea batatas (L.) Lam.]
TCH

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title = "Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]: Molecular cloning and functional analysis",

abstract = "The cDNA of the touch-induced genes (TCH) of the sweet potato [Ipomoea batatas (L.) Lam.] has been cloned and analyzed. IbTCH1, which exists as at least two-copy genes in the genome of the sweet potato, encodes for 148-amino acid polypeptides, and harbors four conversed Ca2+-binding motif EF-hands. IbTCH1 was shown to be expressed in the flower, leaf, thick pigmented root, and particularly in the white fibrous root, but expressed only weakly in the petiole. IbTCH1 is upregulated upon exposure to environmental stresses, dehydration, and jasmonic acid. Furthermore, IbTCH1 is developmentally regulated in the leaf and root. These results strongly indicate that the gene performs functions in both plant development and in defense/stress-signaling pathways.",

keywords = "CaM, Dehydration, Sweet potato [Ipomoea batatas (L.) Lam.], TCH",

author = "Seo, {Sang Gyu} and Kim, {Ji Seong} and Kang, {Seung Won} and Shin, {Mi Rae} and Yang, {You Sun} and Lee, {Gung Pyo} and Hong, {Jin Sung} and Kim, {Sun Hyung}",

year = "2008",

month = nov,

doi = "10.1007/s11816-008-0072-5",

language = "English",

volume = "2",

pages = "253--258",

journal = "Plant Biotechnology Reports",

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T1 - Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]

T2 - Molecular cloning and functional analysis

AU - Seo, Sang Gyu

AU - Kim, Ji Seong

AU - Kang, Seung Won

AU - Shin, Mi Rae

AU - Yang, You Sun

AU - Lee, Gung Pyo

AU - Hong, Jin Sung

AU - Kim, Sun Hyung

PY - 2008/11

Y1 - 2008/11

N2 - The cDNA of the touch-induced genes (TCH) of the sweet potato [Ipomoea batatas (L.) Lam.] has been cloned and analyzed. IbTCH1, which exists as at least two-copy genes in the genome of the sweet potato, encodes for 148-amino acid polypeptides, and harbors four conversed Ca2+-binding motif EF-hands. IbTCH1 was shown to be expressed in the flower, leaf, thick pigmented root, and particularly in the white fibrous root, but expressed only weakly in the petiole. IbTCH1 is upregulated upon exposure to environmental stresses, dehydration, and jasmonic acid. Furthermore, IbTCH1 is developmentally regulated in the leaf and root. These results strongly indicate that the gene performs functions in both plant development and in defense/stress-signaling pathways.

AB - The cDNA of the touch-induced genes (TCH) of the sweet potato [Ipomoea batatas (L.) Lam.] has been cloned and analyzed. IbTCH1, which exists as at least two-copy genes in the genome of the sweet potato, encodes for 148-amino acid polypeptides, and harbors four conversed Ca2+-binding motif EF-hands. IbTCH1 was shown to be expressed in the flower, leaf, thick pigmented root, and particularly in the white fibrous root, but expressed only weakly in the petiole. IbTCH1 is upregulated upon exposure to environmental stresses, dehydration, and jasmonic acid. Furthermore, IbTCH1 is developmentally regulated in the leaf and root. These results strongly indicate that the gene performs functions in both plant development and in defense/stress-signaling pathways.

KW - CaM

KW - Dehydration

KW - Sweet potato [Ipomoea batatas (L.) Lam.]

KW - TCH

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U2 - 10.1007/s11816-008-0072-5

DO - 10.1007/s11816-008-0072-5

M3 - Article

AN - SCOPUS:55949114381

SN - 1863-5466

VL - 2

SP - 253

EP - 258

JO - Plant Biotechnology Reports

JF - Plant Biotechnology Reports

IS - 4

ER -

Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]: Molecular cloning and functional analysis

Abstract

Keywords

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