Validation of a two-generational reproduction test in Daphnia magna: An interlaboratory exercise

Carlos Barata, Bruno Campos, Claudia Rivetti, Gerald A. LeBlanc, Stephanie Eytcheson, Stephanie McKnight, Marysia Tobor-Kaplon, Selinda de Vries Buitenweg, Suhyon Choi, Jinhee Choi, Elena I. Sarapultseva, Marie Agnès Coutellec, Maïra Coke, Pascal Pandard, Arnaud Chaumot, Hervé Quéau, Nicolas Delorme, Olivier Geffard, Fernando Martínez-Jerónimo, Haruna WatanabeNorihisa Tatarazako, Isabel Lopes, João L.T. Pestana, Amadeu M.V.M. Soares, Cecilia Manuela Pereira, Karel De Schamphelaere

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Effects observed within one generation disregard potential detrimental effects that may appear across generations. Previously we have developed a two generation Daphnia magna reproduction test using the OECD TG 211 protocol with a few amendments, including initiating the second generation with third brood neonates produced from first generation individuals. Here we showed the results of an inter-laboratory calibration exercise among 12 partners that aimed to test the robustness and consistency of a two generation Daphnia magna reproduction test. Pyperonyl butoxide (PBO) was used as a test compound. Following experiments, PBO residues were determined by TQD-LC/MS/MS. Chemical analysis denoted minor deviations of measured PBO concentrations in freshly prepared and old test solutions and between real and nominal concentrations in all labs. Other test conditions (water, food, D. magna clone, type of test vessel) varied across partners as allowed in the OECD test guidelines. Cumulative fecundity and intrinsic population growth rates (r) were used to estimate “No observed effect concentrations “NOEC using the solvent control as the control treatment. EC10 and EC-50 values were obtained regression analyses. Eleven of the twelve labs succeeded in meeting the OECD criteria of producing > 60 offspring per female in control treatments during 21 days in each of the two consecutive generations. Analysis of variance partitioning of cumulative fecundity indicated a relatively good performance of most labs with most of the variance accounted for by PBO (56.4%) and PBO by interlaboratory interactions (20.2%), with multigenerational effects within and across PBO concentrations explaining about 6% of the variance. EC50 values for reproduction and population growth rates were on average 16.6 and 20.8% lower among second generation individuals, respectively. In summary these results suggest that the proposed assay is reproducible but cumulative toxicity in the second generation cannot reliably be detected with this assay.

Original languageEnglish
Pages (from-to)1073-1083
Number of pages11
JournalScience of the Total Environment
Volume579
DOIs
StatePublished - 1 Feb 2017

Keywords

  • Contaminants
  • Daphnia reproduction
  • Interlaboratory
  • Life-history
  • Multigeneration assay
  • OECD 211
  • Offspring quality

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