TY - JOUR
T1 - Validation of a two-generational reproduction test in Daphnia magna
T2 - An interlaboratory exercise
AU - Barata, Carlos
AU - Campos, Bruno
AU - Rivetti, Claudia
AU - LeBlanc, Gerald A.
AU - Eytcheson, Stephanie
AU - McKnight, Stephanie
AU - Tobor-Kaplon, Marysia
AU - de Vries Buitenweg, Selinda
AU - Choi, Suhyon
AU - Choi, Jinhee
AU - Sarapultseva, Elena I.
AU - Coutellec, Marie Agnès
AU - Coke, Maïra
AU - Pandard, Pascal
AU - Chaumot, Arnaud
AU - Quéau, Hervé
AU - Delorme, Nicolas
AU - Geffard, Olivier
AU - Martínez-Jerónimo, Fernando
AU - Watanabe, Haruna
AU - Tatarazako, Norihisa
AU - Lopes, Isabel
AU - Pestana, João L.T.
AU - Soares, Amadeu M.V.M.
AU - Pereira, Cecilia Manuela
AU - De Schamphelaere, Karel
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Effects observed within one generation disregard potential detrimental effects that may appear across generations. Previously we have developed a two generation Daphnia magna reproduction test using the OECD TG 211 protocol with a few amendments, including initiating the second generation with third brood neonates produced from first generation individuals. Here we showed the results of an inter-laboratory calibration exercise among 12 partners that aimed to test the robustness and consistency of a two generation Daphnia magna reproduction test. Pyperonyl butoxide (PBO) was used as a test compound. Following experiments, PBO residues were determined by TQD-LC/MS/MS. Chemical analysis denoted minor deviations of measured PBO concentrations in freshly prepared and old test solutions and between real and nominal concentrations in all labs. Other test conditions (water, food, D. magna clone, type of test vessel) varied across partners as allowed in the OECD test guidelines. Cumulative fecundity and intrinsic population growth rates (r) were used to estimate “No observed effect concentrations “NOEC using the solvent control as the control treatment. EC10 and EC-50 values were obtained regression analyses. Eleven of the twelve labs succeeded in meeting the OECD criteria of producing > 60 offspring per female in control treatments during 21 days in each of the two consecutive generations. Analysis of variance partitioning of cumulative fecundity indicated a relatively good performance of most labs with most of the variance accounted for by PBO (56.4%) and PBO by interlaboratory interactions (20.2%), with multigenerational effects within and across PBO concentrations explaining about 6% of the variance. EC50 values for reproduction and population growth rates were on average 16.6 and 20.8% lower among second generation individuals, respectively. In summary these results suggest that the proposed assay is reproducible but cumulative toxicity in the second generation cannot reliably be detected with this assay.
AB - Effects observed within one generation disregard potential detrimental effects that may appear across generations. Previously we have developed a two generation Daphnia magna reproduction test using the OECD TG 211 protocol with a few amendments, including initiating the second generation with third brood neonates produced from first generation individuals. Here we showed the results of an inter-laboratory calibration exercise among 12 partners that aimed to test the robustness and consistency of a two generation Daphnia magna reproduction test. Pyperonyl butoxide (PBO) was used as a test compound. Following experiments, PBO residues were determined by TQD-LC/MS/MS. Chemical analysis denoted minor deviations of measured PBO concentrations in freshly prepared and old test solutions and between real and nominal concentrations in all labs. Other test conditions (water, food, D. magna clone, type of test vessel) varied across partners as allowed in the OECD test guidelines. Cumulative fecundity and intrinsic population growth rates (r) were used to estimate “No observed effect concentrations “NOEC using the solvent control as the control treatment. EC10 and EC-50 values were obtained regression analyses. Eleven of the twelve labs succeeded in meeting the OECD criteria of producing > 60 offspring per female in control treatments during 21 days in each of the two consecutive generations. Analysis of variance partitioning of cumulative fecundity indicated a relatively good performance of most labs with most of the variance accounted for by PBO (56.4%) and PBO by interlaboratory interactions (20.2%), with multigenerational effects within and across PBO concentrations explaining about 6% of the variance. EC50 values for reproduction and population growth rates were on average 16.6 and 20.8% lower among second generation individuals, respectively. In summary these results suggest that the proposed assay is reproducible but cumulative toxicity in the second generation cannot reliably be detected with this assay.
KW - Contaminants
KW - Daphnia reproduction
KW - Interlaboratory
KW - Life-history
KW - Multigeneration assay
KW - OECD 211
KW - Offspring quality
UR - http://www.scopus.com/inward/record.url?scp=85007361078&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2016.11.066
DO - 10.1016/j.scitotenv.2016.11.066
M3 - Article
C2 - 27908627
AN - SCOPUS:85007361078
SN - 0048-9697
VL - 579
SP - 1073
EP - 1083
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -